1j4x
From Proteopedia
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| - | [[Image:1j4x.jpg|left|200px]] | + | [[Image:1j4x.jpg|left|200px]] |
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| - | '''HUMAN VH1-RELATED DUAL-SPECIFICITY PHOSPHATASE C124S MUTANT-PEPTIDE COMPLEX''' | + | {{Structure |
| + | |PDB= 1j4x |SIZE=350|CAPTION= <scene name='initialview01'>1j4x</scene>, resolution 2.75Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Protein-tyrosine-phosphatase Protein-tyrosine-phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.48 3.1.3.48] | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''HUMAN VH1-RELATED DUAL-SPECIFICITY PHOSPHATASE C124S MUTANT-PEPTIDE COMPLEX''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1J4X is a [ | + | 1J4X is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. This structure supersedes the now removed PDB entry 1F5D. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1J4X OCA]. |
==Reference== | ==Reference== | ||
| - | Structural basis for the recognition of a bisphosphorylated MAP kinase peptide by human VHR protein Phosphatase., Schumacher MA, Todd JL, Rice AE, Tanner KG, Denu JM, Biochemistry. 2002 Mar 5;41(9):3009-17. PMID:[http:// | + | Structural basis for the recognition of a bisphosphorylated MAP kinase peptide by human VHR protein Phosphatase., Schumacher MA, Todd JL, Rice AE, Tanner KG, Denu JM, Biochemistry. 2002 Mar 5;41(9):3009-17. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11863439 11863439] |
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Protein-tyrosine-phosphatase]] | [[Category: Protein-tyrosine-phosphatase]] | ||
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[[Category: protein dual-specificity phosphatase]] | [[Category: protein dual-specificity phosphatase]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:58:54 2008'' |
Revision as of 09:58, 20 March 2008
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| , resolution 2.75Å | |||||||
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| Activity: | Protein-tyrosine-phosphatase, with EC number 3.1.3.48 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
HUMAN VH1-RELATED DUAL-SPECIFICITY PHOSPHATASE C124S MUTANT-PEPTIDE COMPLEX
Overview
Human VHR (vaccinia H1 related phosphatase) is a member of the dual-specificity phosphatases (DSPs) that often act on bisphosphorylated protein substrates. Unlike most DSPs, VHR displays a strong preference for dephosphorylating phosphotyrosine residues over phosphothreonine residues. Here we describe the 2.75 A crystal structure of the C124S inactive VHR mutant in complex with a bisphosphorylated peptide corresponding to the MAP kinase activation lip. This structure and subsequent biochemical studies revealed the basis for the strong preference for hydrolyzing phosphotyrosine within bisphosphorylated substrates containing -pTXpY-. In the structure, the two phospho residues are oriented into distinct pockets; the phosphotyrosine is bound in the exposed yet deep active site cleft while the phosphothreonine is loosely tethered into a nearby basic pocket containing Arg(158). As this structure is the first substrate-enzyme complex reported for the DSP family of enzymes, these results provide the first glimpse into how DSPs bind their protein substrates.
About this Structure
1J4X is a Single protein structure of sequence from Homo sapiens. This structure supersedes the now removed PDB entry 1F5D. Full crystallographic information is available from OCA.
Reference
Structural basis for the recognition of a bisphosphorylated MAP kinase peptide by human VHR protein Phosphatase., Schumacher MA, Todd JL, Rice AE, Tanner KG, Denu JM, Biochemistry. 2002 Mar 5;41(9):3009-17. PMID:11863439
Page seeded by OCA on Thu Mar 20 11:58:54 2008
