2nap

From Proteopedia

Revision as of 15:31, 30 October 2007

DISSIMILATORY NITRATE REDUCTASE (NAP) FROM DESULFOVIBRIO DESULFURICANS

Overview

BACKGROUND: The periplasmic nitrate reductase (NAP) from the sulphate, reducing bacterium Desulfovibrio desulfuricans ATCC 27774 is induced by, growth on nitrate and catalyses the reduction of nitrate to nitrite for, respiration. NAP is a molybdenum-containing enzyme with one, bis-molybdopterin guanine dinucleotide (MGD) cofactor and one [4Fe-4S], cluster in a single polypeptide chain of 723 amino acid residues. To date, there is no crystal structure of a nitrate reductase. RESULTS: The first, crystal structure of a dissimilatory (respiratory) nitrate reductase was, determined at 1.9 A resolution by multiwavelength anomalous diffraction, (MAD) methods. The structure is folded into four domains with an, alpha/beta-type topology and all four domains are involved in cofactor, binding. The ... [(full description)]

About this Structure

2NAP is a [Single protein] structure of sequence from [Desulfovibrio desulfuricans] with SF4, MO, MGD and MES as [ligands]. Structure known Active Sites: FS4 and MGD. Full crystallographic information is available from [OCA].

Reference

Crystal structure of the first dissimilatory nitrate reductase at 1.9 A solved by MAD methods., Dias JM, Than ME, Humm A, Huber R, Bourenkov GP, Bartunik HD, Bursakov S, Calvete J, Caldeira J, Carneiro C, Moura JJ, Moura I, Romao MJ, Structure. 1999 Jan 15;7(1):65-79. PMID:10368307[[Category: [4fe-4s] cluster]]

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