1jeq
From Proteopedia
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| - | [[Image:1jeq.jpg|left|200px]] | + | [[Image:1jeq.jpg|left|200px]] |
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| - | '''Crystal Structure of the Ku Heterodimer''' | + | {{Structure |
| + | |PDB= 1jeq |SIZE=350|CAPTION= <scene name='initialview01'>1jeq</scene>, resolution 2.70Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= G22P1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]), XRCC5 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]) | ||
| + | }} | ||
| + | |||
| + | '''Crystal Structure of the Ku Heterodimer''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1JEQ is a [ | + | 1JEQ is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JEQ OCA]. |
==Reference== | ==Reference== | ||
| - | Structure of the Ku heterodimer bound to DNA and its implications for double-strand break repair., Walker JR, Corpina RA, Goldberg J, Nature. 2001 Aug 9;412(6847):607-14. PMID:[http:// | + | Structure of the Ku heterodimer bound to DNA and its implications for double-strand break repair., Walker JR, Corpina RA, Goldberg J, Nature. 2001 Aug 9;412(6847):607-14. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11493912 11493912] |
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
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[[Category: sap domain]] | [[Category: sap domain]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:02:24 2008'' |
Revision as of 10:02, 20 March 2008
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| , resolution 2.70Å | |||||||
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| Gene: | G22P1 (Homo sapiens), XRCC5 (Homo sapiens) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal Structure of the Ku Heterodimer
Overview
The Ku heterodimer (Ku70 and Ku80 subunits) contributes to genomic integrity through its ability to bind DNA double-strand breaks and facilitate repair by the non-homologous end-joining pathway. The crystal structure of the human Ku heterodimer was determined both alone and bound to a 55-nucleotide DNA element at 2.7 and 2.5 A resolution, respectively. Ku70 and Ku80 share a common topology and form a dyad-symmetrical molecule with a preformed ring that encircles duplex DNA. The binding site can cradle two full turns of DNA while encircling only the central 3-4 base pairs (bp). Ku makes no contacts with DNA bases and few with the sugar-phosphate backbone, but it fits sterically to major and minor groove contours so as to position the DNA helix in a defined path through the protein ring. These features seem well designed to structurally support broken DNA ends and to bring the DNA helix into phase across the junction during end processing and ligation.
About this Structure
1JEQ is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Structure of the Ku heterodimer bound to DNA and its implications for double-strand break repair., Walker JR, Corpina RA, Goldberg J, Nature. 2001 Aug 9;412(6847):607-14. PMID:11493912
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