1qfd

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[[Image:1qfd.png|left|200px]]
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==NMR SOLUTION STRUCTURE OF ALPHA-AMYLASE INHIBITOR (AAI)==
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<StructureSection load='1qfd' size='340' side='right' caption='[[1qfd]], [[NMR_Ensembles_of_Models | 10 NMR models]]' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1qfd]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Amaranthus_hypochondriacus Amaranthus hypochondriacus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QFD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1QFD FirstGlance]. <br>
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</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1qfd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qfd OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1qfd RCSB], [http://www.ebi.ac.uk/pdbsum/1qfd PDBsum]</span></td></tr>
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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alpha-Amylase inhibitor (AAI), a 32-residue miniprotein from the Mexican crop plant amaranth (Amaranthus hypochondriacus), is the smallest known alpha-amylase inhibitor and is specific for insect alpha-amylases (Chagolla-Lopez, A., Blanco-Labra, A., Patthy, A., Sanchez, R., and Pongor, S. (1994) J. Biol. Chem. 269, 23675-23680). Its disulfide topology was confirmed by Edman degradation, and its three-dimensional solution structure was determined by two-dimensional 1H NMR spectroscopy at 500 MHz. Structural constraints (consisting of 348 nuclear Overhauser effect interproton distances, 8 backbone dihedral constraints, and 9 disulfide distance constraints) were used as an input to the X-PLOR program for simulated annealing and energy minimization calculations. The final set of 10 structures had a mean pairwise root mean square deviation of 0.32 A for the backbone atoms and 1.04 A for all heavy atoms. The structure of AAI consists of a short triple-stranded beta-sheet stabilized by three disulfide bonds, forming a typical knottin or inhibitor cystine knot fold found in miniproteins, which binds various macromolecular ligands. When the first intercystine segment of AAI (sequence IPKWNR) was inserted into a homologous position of the spider toxin Huwentoxin I, the resulting chimera showed a significant inhibitory activity, suggesting that this segment takes part in enzyme binding.
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{{STRUCTURE_1qfd| PDB=1qfd | SCENE= }}
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Solution structure of the major alpha-amylase inhibitor of the crop plant amaranth.,Lu S, Deng P, Liu X, Luo J, Han R, Gu X, Liang S, Wang X, Li F, Lozanov V, Patthy A, Pongor S J Biol Chem. 1999 Jul 16;274(29):20473-8. PMID:10400675<ref>PMID:10400675</ref>
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===NMR SOLUTION STRUCTURE OF ALPHA-AMYLASE INHIBITOR (AAI)===
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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{{ABSTRACT_PUBMED_10400675}}
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== References ==
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<references/>
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==About this Structure==
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__TOC__
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[[1qfd]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Amaranthus_hypochondriacus Amaranthus hypochondriacus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QFD OCA].
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</StructureSection>
[[Category: Amaranthus hypochondriacus]]
[[Category: Amaranthus hypochondriacus]]
[[Category: Deng, P.]]
[[Category: Deng, P.]]

Revision as of 08:17, 8 October 2014

NMR SOLUTION STRUCTURE OF ALPHA-AMYLASE INHIBITOR (AAI)

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