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1jf9
From Proteopedia
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| - | [[Image:1jf9.gif|left|200px]] | + | [[Image:1jf9.gif|left|200px]] |
| - | + | ||
| - | '''Crystal Structure of selenocysteine lyase''' | + | {{Structure |
| + | |PDB= 1jf9 |SIZE=350|CAPTION= <scene name='initialview01'>1jf9</scene>, resolution 2.Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=PLP:PYRIDOXAL-5'-PHOSPHATE'>PLP</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Selenocysteine_lyase Selenocysteine lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.4.1.16 4.4.1.16] | ||
| + | |GENE= NIFS/CSDB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli]) | ||
| + | }} | ||
| + | |||
| + | '''Crystal Structure of selenocysteine lyase''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1JF9 is a [ | + | 1JF9 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JF9 OCA]. |
==Reference== | ==Reference== | ||
| - | Analysis of the E. coli NifS CsdB protein at 2.0 A reveals the structural basis for perselenide and persulfide intermediate formation., Lima CD, J Mol Biol. 2002 Feb 1;315(5):1199-208. PMID:[http:// | + | Analysis of the E. coli NifS CsdB protein at 2.0 A reveals the structural basis for perselenide and persulfide intermediate formation., Lima CD, J Mol Biol. 2002 Feb 1;315(5):1199-208. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11827487 11827487] |
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Selenocysteine lyase]] | [[Category: Selenocysteine lyase]] | ||
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[[Category: cysteine]] | [[Category: cysteine]] | ||
[[Category: new york structural genomix research consortium]] | [[Category: new york structural genomix research consortium]] | ||
| - | [[Category: | + | [[Category: nif]] |
[[Category: nysgxrc]] | [[Category: nysgxrc]] | ||
[[Category: protein structure initiative]] | [[Category: protein structure initiative]] | ||
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[[Category: pursulfide]] | [[Category: pursulfide]] | ||
[[Category: selenocysteine]] | [[Category: selenocysteine]] | ||
| - | [[Category: structural | + | [[Category: structural genomic]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:02:40 2008'' |
Revision as of 10:02, 20 March 2008
| |||||||
| , resolution 2.Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Gene: | NIFS/CSDB (Escherichia coli) | ||||||
| Activity: | Selenocysteine lyase, with EC number 4.4.1.16 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal Structure of selenocysteine lyase
Overview
The Escherichia coli NifS CsdB protein is a member of the homodimeric pyridoxal 5'-phosphate (PLP)-dependent family of enzymes. These enzymes are capable of decomposing cysteine or selenocysteine into L-alanine and sulfur or selenium, respectively. E. coli NifS CsdB has a high specificity for L-selenocysteine in comparison to l-cysteine, suggesting a role for this enzyme is selenium metabolism. The 2.0 A crystal structure of E. coli NifS CsdB reveals a high-resolution view of the active site of this enzyme in apo-, persulfide, perselenide, and selenocysteine-bound intermediates, suggesting a mechanism for the stabilization of the enzyme persulfide and perselenide intermediates during catalysis, a necessary intermediate in the formation of sulfur and selenium containing metabolites.
About this Structure
1JF9 is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Analysis of the E. coli NifS CsdB protein at 2.0 A reveals the structural basis for perselenide and persulfide intermediate formation., Lima CD, J Mol Biol. 2002 Feb 1;315(5):1199-208. PMID:11827487
Page seeded by OCA on Thu Mar 20 12:02:40 2008
Categories: Escherichia coli | Selenocysteine lyase | Single protein | Burley, S K. | Lima, C D. | NYSGXRC, New York Structural GenomiX Research Consortium. | PLP | Cysteine | New york structural genomix research consortium | Nif | Nysgxrc | Protein structure initiative | Psi | Pursulfide | Selenocysteine | Structural genomic
