1jf9

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[[Image:1jf9.gif|left|200px]]<br /><applet load="1jf9" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1jf9.gif|left|200px]]
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caption="1jf9, resolution 2.&Aring;" />
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'''Crystal Structure of selenocysteine lyase'''<br />
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{{Structure
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|PDB= 1jf9 |SIZE=350|CAPTION= <scene name='initialview01'>1jf9</scene>, resolution 2.&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=PLP:PYRIDOXAL-5'-PHOSPHATE'>PLP</scene>
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|ACTIVITY= [http://en.wikipedia.org/wiki/Selenocysteine_lyase Selenocysteine lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.4.1.16 4.4.1.16]
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|GENE= NIFS/CSDB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
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}}
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'''Crystal Structure of selenocysteine lyase'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1JF9 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=PLP:'>PLP</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Selenocysteine_lyase Selenocysteine lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.4.1.16 4.4.1.16] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JF9 OCA].
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1JF9 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JF9 OCA].
==Reference==
==Reference==
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Analysis of the E. coli NifS CsdB protein at 2.0 A reveals the structural basis for perselenide and persulfide intermediate formation., Lima CD, J Mol Biol. 2002 Feb 1;315(5):1199-208. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11827487 11827487]
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Analysis of the E. coli NifS CsdB protein at 2.0 A reveals the structural basis for perselenide and persulfide intermediate formation., Lima CD, J Mol Biol. 2002 Feb 1;315(5):1199-208. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11827487 11827487]
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
[[Category: Selenocysteine lyase]]
[[Category: Selenocysteine lyase]]
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[[Category: cysteine]]
[[Category: cysteine]]
[[Category: new york structural genomix research consortium]]
[[Category: new york structural genomix research consortium]]
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[[Category: nifs]]
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[[Category: nif]]
[[Category: nysgxrc]]
[[Category: nysgxrc]]
[[Category: protein structure initiative]]
[[Category: protein structure initiative]]
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[[Category: pursulfide]]
[[Category: pursulfide]]
[[Category: selenocysteine]]
[[Category: selenocysteine]]
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[[Category: structural genomics]]
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[[Category: structural genomic]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:22:00 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:02:40 2008''

Revision as of 10:02, 20 March 2008

Image:1jf9.gif


PDB ID 1jf9

Drag the structure with the mouse to rotate
, resolution 2.Å
Ligands:
Gene: NIFS/CSDB (Escherichia coli)
Activity: Selenocysteine lyase, with EC number 4.4.1.16
Coordinates: save as pdb, mmCIF, xml



Crystal Structure of selenocysteine lyase


Overview

The Escherichia coli NifS CsdB protein is a member of the homodimeric pyridoxal 5'-phosphate (PLP)-dependent family of enzymes. These enzymes are capable of decomposing cysteine or selenocysteine into L-alanine and sulfur or selenium, respectively. E. coli NifS CsdB has a high specificity for L-selenocysteine in comparison to l-cysteine, suggesting a role for this enzyme is selenium metabolism. The 2.0 A crystal structure of E. coli NifS CsdB reveals a high-resolution view of the active site of this enzyme in apo-, persulfide, perselenide, and selenocysteine-bound intermediates, suggesting a mechanism for the stabilization of the enzyme persulfide and perselenide intermediates during catalysis, a necessary intermediate in the formation of sulfur and selenium containing metabolites.

About this Structure

1JF9 is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Analysis of the E. coli NifS CsdB protein at 2.0 A reveals the structural basis for perselenide and persulfide intermediate formation., Lima CD, J Mol Biol. 2002 Feb 1;315(5):1199-208. PMID:11827487

Page seeded by OCA on Thu Mar 20 12:02:40 2008

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