2jyk

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[[Image:2jyk.png|left|200px]]
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==NMR Structure of a 21 bp DNA duplex preferentially cleaved by Human Topoisomerase II==
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<StructureSection load='2jyk' size='340' side='right' caption='[[2jyk]], [[NMR_Ensembles_of_Models | 10 NMR models]]' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2jyk]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JYK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2JYK FirstGlance]. <br>
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</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2jyk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jyk OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2jyk RCSB], [http://www.ebi.ac.uk/pdbsum/2jyk PDBsum]</span></td></tr>
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Topoisomerase II enzymes are essential enzymes that modulate DNA topology and play a role in chromatin compaction. While these enzymes appear to recognize and cleave the DNA in a nonrandom fashion, factors that underlie enzyme specificity remain an enigma. To gain new insights on these topics, we undertake, using NMR and molecular dynamics methods, studies of the structural and dynamic features of a 21 bp DNA segment preferentially cleaved by topoisomerases II. The large size of the oligonucleotide did not hamper the determination of structures of sufficient quality, and numerous interesting correlations between helicoidal parameters already depicted in crystals and molecular dynamics simulations are recovered here. The main feature of the sequence is the occurrence of a large opening of the base pairs in a four-residue AT-rich region located immediately at the 5' end of one of the cleaved sites. This opening seems to be largely dependent on sequence context, since a similar opening is not found in the other AT base pairs of the sequence. Furthermore, two adenine nucleotides of the same portion of the oligonucleotide present slow internal motions at the NMR timescale, revealing particular base dynamics. In conclusion, this AT-rich region presents the most salient character in the sequence and could be involved in the preferential cleavage by topoisomerase II. The examination of preferred sites in the literature pointed out the frequent occurrence of AT-rich sequences, namely matrix attachment region and scaffold attachment region sequences, at the sites cleaved by topoisomerase II. We could infer that the particular flexibility of these sequences plays an important role in enabling the formation of a competent cleavage complex. The sequences could then be selected based on their facility to undertake conformational change during the complex formation, rather than purely based on binding affinity.
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{{STRUCTURE_2jyk| PDB=2jyk | SCENE= }}
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Identification of Intrinsic Dynamics in a DNA Sequence Preferentially Cleaved by Topoisomerase II Enzyme.,Masliah G, Rene B, Zargarian L, Fermandjian S, Mauffret O J Mol Biol. 2008 Jun 17;. PMID:18585388<ref>PMID:18585388</ref>
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===NMR Structure of a 21 bp DNA duplex preferentially cleaved by Human Topoisomerase II===
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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{{ABSTRACT_PUBMED_18585388}}
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== References ==
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<references/>
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==About this Structure==
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__TOC__
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[[2jyk]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JYK OCA].
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</StructureSection>
[[Category: Masliah, G.]]
[[Category: Masliah, G.]]
[[Category: Mauffret, O.]]
[[Category: Mauffret, O.]]

Revision as of 15:29, 12 October 2014

NMR Structure of a 21 bp DNA duplex preferentially cleaved by Human Topoisomerase II

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