This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


2idn

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
m (Protected "2idn" [edit=sysop:move=sysop])
Line 1: Line 1:
-
[[Image:2idn.png|left|200px]]
+
==NMR structure of a new modified Thrombin Binding Aptamer containing a 5'-5' inversion of polarity site==
 +
<StructureSection load='2idn' size='340' side='right' caption='[[2idn]], [[NMR_Ensembles_of_Models | 7 NMR models]]' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[2idn]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IDN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2IDN FirstGlance]. <br>
 +
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2idn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2idn OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2idn RCSB], [http://www.ebi.ac.uk/pdbsum/2idn PDBsum]</span></td></tr>
 +
</table>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
The solution structure of a new modified thrombin binding aptamer (TBA) containing a 5'-5' inversion of polarity site, namely d(3'GGT5'-5'TGGTGTGGTTGG3'), is reported. NMR and CD spectroscopy, as well as molecular dynamic and mechanic calculations, have been used to characterize the 3D structure. The modified oligonucleotide is characterized by a chair-like structure consisting of two G-tetrads connected by three edge-wise TT, TGT and TT loops. d(3'GGT5'-5'TGGTGTGGTTGG3') is characterized by an unusual folding, being three strands parallel to each other and only one strand oriented in opposite manner. This led to an anti-anti-anti-syn and syn-syn-syn-anti arrangement of the Gs in the two tetrads. The thermal stability of the modified oligonucleotide is 4 degrees C higher than the corresponding unmodified TBA. d(3'GGT5'-5'TGGTGTGGTTGG3') continues to display an anticoagulant activity, even if decreased with respect to the TBA.
-
{{STRUCTURE_2idn| PDB=2idn | SCENE= }}
+
A new modified thrombin binding aptamer containing a 5'-5' inversion of polarity site.,Martino L, Virno A, Randazzo A, Virgilio A, Esposito V, Giancola C, Bucci M, Cirino G, Mayol L Nucleic Acids Res. 2006;34(22):6653-62. Epub 2006 Dec 1. PMID:17145716<ref>PMID:17145716</ref>
-
===NMR structure of a new modified Thrombin Binding Aptamer containing a 5'-5' inversion of polarity site===
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
 
+
</div>
-
{{ABSTRACT_PUBMED_17145716}}
+
== References ==
-
 
+
<references/>
-
==About this Structure==
+
__TOC__
-
[[2idn]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IDN OCA].
+
</StructureSection>
[[Category: Giancola, C.]]
[[Category: Giancola, C.]]
[[Category: Martino, L.]]
[[Category: Martino, L.]]

Revision as of 15:38, 12 October 2014

NMR structure of a new modified Thrombin Binding Aptamer containing a 5'-5' inversion of polarity site

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2idn"
Personal tools