1jo2
From Proteopedia
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| - | [[Image:1jo2.gif|left|200px]] | + | [[Image:1jo2.gif|left|200px]] |
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| - | '''Crystal Structure of the B-DNA Hexamer (CgATCG).Daunomycin Complex Containing a Ribose at the Intercalation Site''' | + | {{Structure |
| + | |PDB= 1jo2 |SIZE=350|CAPTION= <scene name='initialview01'>1jo2</scene>, resolution 1.50Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=DM1:DAUNOMYCIN'>DM1</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''Crystal Structure of the B-DNA Hexamer (CgATCG).Daunomycin Complex Containing a Ribose at the Intercalation Site''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1JO2 is a [ | + | 1JO2 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JO2 OCA]. |
==Reference== | ==Reference== | ||
| - | Structure of a B-form DNA/RNA chimera (dC)(rG)d(ATCG) complexed with daunomycin at 1.5 A resolution., Shi K, Pan B, Sundaralingam M, Acta Crystallogr D Biol Crystallogr. 2003 Aug;59(Pt 8):1377-83. Epub 2003, Jul 23. PMID:[http:// | + | Structure of a B-form DNA/RNA chimera (dC)(rG)d(ATCG) complexed with daunomycin at 1.5 A resolution., Shi K, Pan B, Sundaralingam M, Acta Crystallogr D Biol Crystallogr. 2003 Aug;59(Pt 8):1377-83. Epub 2003, Jul 23. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12876339 12876339] |
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Pan, B.]] | [[Category: Pan, B.]] | ||
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[[Category: intercalation]] | [[Category: intercalation]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:06:02 2008'' |
Revision as of 10:06, 20 March 2008
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| , resolution 1.50Å | |||||||
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| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal Structure of the B-DNA Hexamer (CgATCG).Daunomycin Complex Containing a Ribose at the Intercalation Site
Overview
The crystal structure of a DNA/RNA chimera (dC)(rG)d(ATCG) complexed with the anticancer drug daunomycin has been determined at 1.5 A resolution with R(work) and R(free) of 19.7 and 23.3%, respectively, for 2767 reflections. The complex crystallizes in space group P4(1)2(1)2, with unit-cell parameters a = b = 28.05, c = 53.16 A, and contains one nucleic acid strand and one daunomycin molecule in the asymmetric unit. To our knowledge, this is the first crystal structure of a DNA/RNA chimera complexed with an intercalating drug. The DNA/RNA chimera adopts the B-form helical conformation, with the 2'-hydroxyl group in the major groove of the duplex, forming hydrogen bonds to N7 and the anionic phosphate oxygen of its 3'-side adenine. The present results indicate that the replacement by the ribose sugar in the DNA sequence does not change the geometry and intercalation pattern of daunomycin. A model of B-form RNA has been built based on the present structure. The model indicates that the interactions of the 2'-hydroxyl groups in the B-form duplex depend on their 3'-side nucleotides.
About this Structure
1JO2 is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
Structure of a B-form DNA/RNA chimera (dC)(rG)d(ATCG) complexed with daunomycin at 1.5 A resolution., Shi K, Pan B, Sundaralingam M, Acta Crystallogr D Biol Crystallogr. 2003 Aug;59(Pt 8):1377-83. Epub 2003, Jul 23. PMID:12876339
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