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1jqy

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[[Image:1jqy.gif|left|200px]]<br /><applet load="1jqy" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1jqy.gif|left|200px]]
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caption="1jqy, resolution 2.1400&Aring;" />
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'''HEAT-LABILE ENTEROTOXIN B-PENTAMER WITH LIGAND BMSC-0010'''<br />
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{{Structure
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|PDB= 1jqy |SIZE=350|CAPTION= <scene name='initialview01'>1jqy</scene>, resolution 2.1400&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=A32:(3-NITRO-5-(3-MORPHOLIN-4-YL-PROPYLAMINOCARBONYL)PHENYL)-GALACTOPYRANOSIDE'>A32</scene>
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|ACTIVITY=
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|GENE= ETXB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
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}}
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'''HEAT-LABILE ENTEROTOXIN B-PENTAMER WITH LIGAND BMSC-0010'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1JQY is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=A32:'>A32</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JQY OCA].
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1JQY is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JQY OCA].
==Reference==
==Reference==
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Anchor-based design of improved cholera toxin and E. coli heat-labile enterotoxin receptor binding antagonists that display multiple binding modes., Pickens JC, Merritt EA, Ahn M, Verlinde CL, Hol WG, Fan E, Chem Biol. 2002 Feb;9(2):215-24. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11880036 11880036]
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Anchor-based design of improved cholera toxin and E. coli heat-labile enterotoxin receptor binding antagonists that display multiple binding modes., Pickens JC, Merritt EA, Ahn M, Verlinde CL, Hol WG, Fan E, Chem Biol. 2002 Feb;9(2):215-24. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11880036 11880036]
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: receptor]]
[[Category: receptor]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:25:42 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:07:13 2008''

Revision as of 10:07, 20 March 2008

Image:1jqy.gif


PDB ID 1jqy

Drag the structure with the mouse to rotate
, resolution 2.1400Å
Ligands:
Gene: ETXB (Escherichia coli)
Coordinates: save as pdb, mmCIF, xml



HEAT-LABILE ENTEROTOXIN B-PENTAMER WITH LIGAND BMSC-0010


Overview

The action of cholera toxin and E. coli heat-labile enterotoxin can be inhibited by blocking their binding to the cell-surface receptor GM1. We have used anchor-based design to create 15 receptor binding inhibitors that contain the previously characterized inhibitor MNPG as a substructure. In ELISA assays, all 15 compounds exhibited increased potency relative to MNPG. Binding affinities for two compounds, each containing a morpholine ring linked to MNPG via a hydrophobic tail, were characterized by pulsed ultrafiltration (PUF) and isothermal titration calorimetry (ITC). Crystal structures for these compounds bound to toxin B pentamer revealed a conserved binding mode for the MNPG moiety, with multiple binding modes adopted by the attached morpholine derivatives. The observed binding interactions can be exploited in the design of improved toxin binding inhibitors.

About this Structure

1JQY is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Anchor-based design of improved cholera toxin and E. coli heat-labile enterotoxin receptor binding antagonists that display multiple binding modes., Pickens JC, Merritt EA, Ahn M, Verlinde CL, Hol WG, Fan E, Chem Biol. 2002 Feb;9(2):215-24. PMID:11880036

Page seeded by OCA on Thu Mar 20 12:07:13 2008

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