1jr3
From Proteopedia
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| - | [[Image:1jr3.jpg|left|200px]] | + | [[Image:1jr3.jpg|left|200px]] |
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| - | '''Crystal Structure of the Processivity Clamp Loader Gamma Complex of E. coli DNA Polymerase III''' | + | {{Structure |
| + | |PDB= 1jr3 |SIZE=350|CAPTION= <scene name='initialview01'>1jr3</scene>, resolution 2.7Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene> and <scene name='pdbligand=SO4:SULFATE ION'>SO4</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/DNA-directed_DNA_polymerase DNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.7 2.7.7.7] | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''Crystal Structure of the Processivity Clamp Loader Gamma Complex of E. coli DNA Polymerase III''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1JR3 is a [ | + | 1JR3 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JR3 OCA]. |
==Reference== | ==Reference== | ||
| - | Crystal structure of the processivity clamp loader gamma (gamma) complex of E. coli DNA polymerase III., Jeruzalmi D, O'Donnell M, Kuriyan J, Cell. 2001 Aug 24;106(4):429-41. PMID:[http:// | + | Crystal structure of the processivity clamp loader gamma (gamma) complex of E. coli DNA polymerase III., Jeruzalmi D, O'Donnell M, Kuriyan J, Cell. 2001 Aug 24;106(4):429-41. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11525729 11525729] |
[[Category: DNA-directed DNA polymerase]] | [[Category: DNA-directed DNA polymerase]] | ||
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
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[[Category: processivity clamp]] | [[Category: processivity clamp]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:07:18 2008'' |
Revision as of 10:07, 20 March 2008
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| , resolution 2.7Å | |||||||
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| Ligands: | and | ||||||
| Activity: | DNA-directed DNA polymerase, with EC number 2.7.7.7 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal Structure of the Processivity Clamp Loader Gamma Complex of E. coli DNA Polymerase III
Overview
The gamma complex, an AAA+ ATPase, is the bacterial homolog of eukaryotic replication factor C (RFC) that loads the sliding clamp (beta, homologous to PCNA) onto DNA. The 2.7/3.0 A crystal structure of gamma complex reveals a pentameric arrangement of subunits, with stoichiometry delta':gamma(3):delta. The C-terminal domains of the subunits form a circular collar that supports an asymmetric arrangement of the N-terminal ATP binding domains of the gamma motor and the structurally related domains of the delta' stator and the delta wrench. The structure suggests a mechanism by which the gamma complex switches between a closed state, in which the beta-interacting element of delta is hidden by delta', and an open form similar to the crystal structure, in which delta is free to bind to beta.
About this Structure
1JR3 is a Protein complex structure of sequences from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Crystal structure of the processivity clamp loader gamma (gamma) complex of E. coli DNA polymerase III., Jeruzalmi D, O'Donnell M, Kuriyan J, Cell. 2001 Aug 24;106(4):429-41. PMID:11525729
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