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2ost

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[[Image:2ost.png|left|200px]]
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==The structure of a bacterial homing endonuclease : I-Ssp6803I==
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<StructureSection load='2ost' size='340' side='right' caption='[[2ost]], [[Resolution|resolution]] 3.10&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2ost]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Synechocystis_sp. Synechocystis sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OST OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2OST FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ost FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ost OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2ost RCSB], [http://www.ebi.ac.uk/pdbsum/2ost PDBsum]</span></td></tr>
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The homing endonuclease I-Ssp6803I causes the insertion of a group I intron into a bacterial tRNA gene-the only example of an invasive mobile intron within a bacterial genome. Using a computational fold prediction, mutagenic screen and crystal structure determination, we demonstrate that this protein is a tetrameric PD-(D/E)-XK endonuclease - a fold normally used to protect a bacterial genome from invading DNA through the action of restriction endonucleases. I-Ssp6803I uses its tetrameric assembly to promote recognition of a single long target site, whereas restriction endonuclease tetramers facilitate cooperative binding and cleavage of two short sites. The limited use of the PD-(D/E)-XK nucleases by mobile introns stands in contrast to their frequent use of LAGLIDADG and HNH endonucleases - which in turn, are rarely incorporated into restriction/modification systems.
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{{STRUCTURE_2ost| PDB=2ost | SCENE= }}
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The restriction fold turns to the dark side: a bacterial homing endonuclease with a PD-(D/E)-XK motif.,Zhao L, Bonocora RP, Shub DA, Stoddard BL EMBO J. 2007 May 2;26(9):2432-42. Epub 2007 Apr 5. PMID:17410205<ref>PMID:17410205</ref>
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===The structure of a bacterial homing endonuclease : I-Ssp6803I===
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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{{ABSTRACT_PUBMED_17410205}}
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== References ==
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<references/>
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==About this Structure==
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__TOC__
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[[2ost]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Synechocystis_sp. Synechocystis sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OST OCA].
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</StructureSection>
[[Category: Synechocystis sp.]]
[[Category: Synechocystis sp.]]
[[Category: Bonocora, R P.]]
[[Category: Bonocora, R P.]]

Revision as of 11:18, 20 October 2014

The structure of a bacterial homing endonuclease : I-Ssp6803I

2ost, resolution 3.10Å

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