1jrs
From Proteopedia
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| - | [[Image:1jrs.gif|left|200px]] | + | [[Image:1jrs.gif|left|200px]] |
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| - | '''HEMIACETAL COMPLEX BETWEEN LEUPEPTIN AND TRYPSIN''' | + | {{Structure |
| + | |PDB= 1jrs |SIZE=350|CAPTION= <scene name='initialview01'>1jrs</scene>, resolution 1.8Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene> and <scene name='pdbligand=ACE:ACETYL GROUP'>ACE</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Trypsin Trypsin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.4 3.4.21.4] | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''HEMIACETAL COMPLEX BETWEEN LEUPEPTIN AND TRYPSIN''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1JRS is a [ | + | 1JRS is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JRS OCA]. |
==Reference== | ==Reference== | ||
| - | Two crystal structures of the leupeptin-trypsin complex., Kurinov IV, Harrison RW, Protein Sci. 1996 Apr;5(4):752-8. PMID:[http:// | + | Two crystal structures of the leupeptin-trypsin complex., Kurinov IV, Harrison RW, Protein Sci. 1996 Apr;5(4):752-8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8845765 8845765] |
[[Category: Bos taurus]] | [[Category: Bos taurus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: digestion]] | [[Category: digestion]] | ||
[[Category: hydrolase]] | [[Category: hydrolase]] | ||
| - | [[Category: | + | [[Category: pancrea]] |
[[Category: serine protease]] | [[Category: serine protease]] | ||
[[Category: zymogen]] | [[Category: zymogen]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:07:34 2008'' |
Revision as of 10:07, 20 March 2008
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| , resolution 1.8Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | and | ||||||
| Activity: | Trypsin, with EC number 3.4.21.4 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
HEMIACETAL COMPLEX BETWEEN LEUPEPTIN AND TRYPSIN
Overview
Three-dimensional structures of trypsin with the reversible inhibitor leupeptin have been determined in two different crystal forms. The first structure was determined at 1.7 A resolution with R-factor = 17.7% in the trigonal crystal space group P3(1)21, with unit cell dimensions of a = b = 55.62 A, c = 110.51 A. The second structure was determined at a resolution of 1.8 A with R-factor = 17.5% in the orthorhombic space group P2(1)2(1)2(1), with unit cell dimensions of a = 63.69 A, b = 69.37 A, c = 63.01 A. The overall protein structure is very similar in both crystal forms, with RMS difference for main-chain atoms of 0.27 A. The leupeptin backbone forms four hydrogen bonds with trypsin and a fifth hydrogen bond interaction is mediated by a water molecule. The aldehyde carbonyl of leupeptin forms a covalent bond of 1.42 A length with side-chain oxygen of Ser-195 in the active site. The reaction of trypsin with leupeptin proceeds through the formation of stable tetrahedral complex in which the hemiacetal oxygen atom is pointing out of the oxyanion hole and forming a hydrogen bond with His-57.
About this Structure
1JRS is a Single protein structure of sequence from Bos taurus. Full crystallographic information is available from OCA.
Reference
Two crystal structures of the leupeptin-trypsin complex., Kurinov IV, Harrison RW, Protein Sci. 1996 Apr;5(4):752-8. PMID:8845765
Page seeded by OCA on Thu Mar 20 12:07:34 2008
Categories: Bos taurus | Single protein | Trypsin | Harrison, R W. | Kurinov, I V. | ACE | CA | Digestion | Hydrolase | Pancrea | Serine protease | Zymogen
