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2wkn

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[[Image:2wkn.png|left|200px]]
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==GAMMA LACTAMASE FROM DELFTIA ACIDOVORANS==
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<StructureSection load='2wkn' size='340' side='right' caption='[[2wkn]], [[Resolution|resolution]] 2.08&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2wkn]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Delftia_acidovorans Delftia acidovorans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WKN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2WKN FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Formamidase Formamidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.49 3.5.1.49] </span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2wkn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wkn OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2wkn RCSB], [http://www.ebi.ac.uk/pdbsum/2wkn PDBsum]</span></td></tr>
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</table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wk/2wkn_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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An enzyme from Comomonas acidovorans has been isolated that is specific for the stereospecific hydrolysis of (+)gamma-lactam. This so-called (+)gamma-lactamase has important applications in biotransformation reactions. The enzyme has been crystallized by vapour-phase diffusion using polyethylene glycol 4000 as a precipitant. Addition of a detergent, beta-octylglucoside, was found to be essential for obtaining diffraction-quality crystals. The crystals grow in the space group P1, with unit-cell parameters a = 63.0, b = 93.2, c = 152.4 A, alpha = 104.3, beta = 92.6, gamma = 108.5 degrees, and diffract to 2 A resolution using synchrotron radiation. Native data from these crystals have been collected to 2.4 A.
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{{STRUCTURE_2wkn| PDB=2wkn | SCENE= }}
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Crystallization and preliminary X-ray analysis of a gamma-lactamase.,Gonsalvez IS, Isupov MN, Littlechild JA Acta Crystallogr D Biol Crystallogr. 2001 Feb;57(Pt 2):284-6. PMID:11173481<ref>PMID:11173481</ref>
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===GAMMA LACTAMASE FROM DELFTIA ACIDOVORANS===
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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{{ABSTRACT_PUBMED_11173481}}
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== References ==
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<references/>
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==About this Structure==
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__TOC__
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[[2wkn]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Delftia_acidovorans Delftia acidovorans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WKN OCA].
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</StructureSection>
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==Reference==
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<ref group="xtra">PMID:011173481</ref><references group="xtra"/>
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[[Category: Delftia acidovorans]]
[[Category: Delftia acidovorans]]
[[Category: Formamidase]]
[[Category: Formamidase]]

Revision as of 11:22, 20 October 2014

GAMMA LACTAMASE FROM DELFTIA ACIDOVORANS

2wkn, resolution 2.08Å

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