2lxq
From Proteopedia
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| - | [[ | + | ==Monomeric PilE G-Quadruplex DNA from Neisseria Gonorrhoeae== |
| + | <StructureSection load='2lxq' size='340' side='right' caption='[[2lxq]], [[NMR_Ensembles_of_Models | 12 NMR models]]' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[2lxq]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LXQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2LXQ FirstGlance]. <br> | ||
| + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2lxq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lxq OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2lxq RCSB], [http://www.ebi.ac.uk/pdbsum/2lxq PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Neisseria gonorrhoeae is an obligate human pathogen that can escape immune surveillance through antigenic variation of surface structures such as pili. A G-quadruplex-forming (G4) sequence (5'-G(3)TG(3)TTG(3)TG(3)) located upstream of the N. gonorrhoeae pilin expression locus (pilE) is necessary for initiation of pilin antigenic variation, a recombination-based, high-frequency, diversity-generation system. We have determined NMR-based structures of the all parallel-stranded monomeric and 5' end-stacked dimeric pilE G-quadruplexes in monovalent cation-containing solutions. We demonstrate that the three-layered all parallel-stranded monomeric pilE G-quadruplex containing single-residue double-chain reversal loops, which can be modeled without steric clashes into the 3 nt DNA-binding site of RecA, binds and promotes E. coli RecA-mediated strand exchange in vitro. We discuss how interactions between RecA and monomeric pilE G-quadruplex could facilitate the specialized recombination reactions leading to pilin diversification. | ||
| - | + | RecA-Binding pilE G4 Sequence Essential for Pilin Antigenic Variation Forms Monomeric and 5' End-Stacked Dimeric Parallel G-Quadruplexes.,Kuryavyi V, Cahoon LA, Seifert HS, Patel DJ Structure. 2012 Oct 16. pii: S0969-2126(12)00347-4. doi:, 10.1016/j.str.2012.09.013. PMID:23085077<ref>PMID:23085077</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | == References == | |
| - | + | <references/> | |
| - | == | + | __TOC__ |
| - | + | </StructureSection> | |
[[Category: Kuryavyi, V V.]] | [[Category: Kuryavyi, V V.]] | ||
[[Category: Patel, D J.]] | [[Category: Patel, D J.]] | ||
Revision as of 11:22, 20 October 2014
Monomeric PilE G-Quadruplex DNA from Neisseria Gonorrhoeae
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