1js2
From Proteopedia
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| - | [[Image:1js2.gif|left|200px]] | + | [[Image:1js2.gif|left|200px]] |
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| - | '''Crystal structure of C77S HiPIP: a serine ligated [4Fe-4S} cluster''' | + | {{Structure |
| + | |PDB= 1js2 |SIZE=350|CAPTION= <scene name='initialview01'>1js2</scene>, resolution 1.90Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=SF4:IRON/SULFUR CLUSTER'>SF4</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''Crystal structure of C77S HiPIP: a serine ligated [4Fe-4S} cluster''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1JS2 is a [ | + | 1JS2 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Allochromatium_vinosum Allochromatium vinosum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JS2 OCA]. |
==Reference== | ==Reference== | ||
| - | Crystal structure and stability studies of C77S HiPIP: a serine ligated [4Fe-4S] cluster., Mansy SS, Xiong Y, Hemann C, Hille R, Sundaralingam M, Cowan JA, Biochemistry. 2002 Jan 29;41(4):1195-201. PMID:[http:// | + | Crystal structure and stability studies of C77S HiPIP: a serine ligated [4Fe-4S] cluster., Mansy SS, Xiong Y, Hemann C, Hille R, Sundaralingam M, Cowan JA, Biochemistry. 2002 Jan 29;41(4):1195-201. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11802718 11802718] |
[[Category: Allochromatium vinosum]] | [[Category: Allochromatium vinosum]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: serine ligated [4fe-4s] cluster]] | [[Category: serine ligated [4fe-4s] cluster]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:07:43 2008'' |
Revision as of 10:07, 20 March 2008
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| , resolution 1.90Å | |||||||
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| Ligands: | |||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of C77S HiPIP: a serine ligated [4Fe-4S} cluster
Overview
The crystal structure of Chromatium vinosum C77S HiPIP has been determined and is compared with that of wild type. This is the first reported crystal structure of a Ser ligated [4Fe-4S] cluster and reveals a 0.11 A shortening of the Fe-O bond (relative to Fe-S), but only minor structural alterations of the overall tertiary structure. Coordination changes are corroborated by resonance Raman spectroscopy. Comparison of the crystal and solution structures for HiPIPs identifies Phe48 as the main controller of solvent access to the Fe-S cluster; however, there is no significant change in cluster solvation of the C77S mutant relative to WT HiPIP. Ser ligation ultimately results in decreased cluster stability due to increased sensitivity to proton mediated degradation.
About this Structure
1JS2 is a Single protein structure of sequence from Allochromatium vinosum. Full crystallographic information is available from OCA.
Reference
Crystal structure and stability studies of C77S HiPIP: a serine ligated [4Fe-4S] cluster., Mansy SS, Xiong Y, Hemann C, Hille R, Sundaralingam M, Cowan JA, Biochemistry. 2002 Jan 29;41(4):1195-201. PMID:11802718[[Category: serine ligated [4fe-4s] cluster]]
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