2kyd

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[[Image:2kyd.png|left|200px]]
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==RDC and RCSA refinement of an A-form RNA: Improvements in Major Groove Width==
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<StructureSection load='2kyd' size='340' side='right' caption='[[2kyd]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2kyd]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KYD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2KYD FirstGlance]. <br>
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</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2kyd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kyd OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2kyd RCSB], [http://www.ebi.ac.uk/pdbsum/2kyd PDBsum]</span></td></tr>
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Ribonucleic acid structure determination by NMR spectroscopy relies primarily on local structural restraints provided by (1)H- (1)H NOEs and J-couplings. When employed loosely, these restraints are broadly compatible with A- and B-like helical geometries and give rise to calculated structures that are highly sensitive to the force fields employed during refinement. A survey of recently reported NMR structures reveals significant variations in helical parameters, particularly the major groove width. Although helical parameters observed in high-resolution X-ray crystal structures of isolated A-form RNA helices are sensitive to crystal packing effects, variations among the published X-ray structures are significantly smaller than those observed in NMR structures. Here we show that restraints derived from aromatic (1)H- (13)C residual dipolar couplings (RDCs) and residual chemical shift anisotropies (RCSAs) can overcome NMR restraint and force field deficiencies and afford structures with helical properties similar to those observed in high-resolution X-ray structures.
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{{STRUCTURE_2kyd| PDB=2kyd | SCENE= }}
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Major groove width variations in RNA structures determined by NMR and impact of 13C residual chemical shift anisotropy and 1H-13C residual dipolar coupling on refinement.,Tolbert BS, Miyazaki Y, Barton S, Kinde B, Starck P, Singh R, Bax A, Case DA, Summers MF J Biomol NMR. 2010 Jul;47(3):205-19. Epub 2010 Jun 15. PMID:20549304<ref>PMID:20549304</ref>
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===RDC and RCSA refinement of an A-form RNA: Improvements in Major Groove Width===
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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{{ABSTRACT_PUBMED_20549304}}
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== References ==
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<references/>
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==About this Structure==
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__TOC__
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[[2kyd]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KYD OCA].
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</StructureSection>
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==Reference==
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<ref group="xtra">PMID:020549304</ref><references group="xtra"/>
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[[Category: Barton, S.]]
[[Category: Barton, S.]]
[[Category: Bax, A.]]
[[Category: Bax, A.]]

Revision as of 11:29, 20 October 2014

RDC and RCSA refinement of an A-form RNA: Improvements in Major Groove Width

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