2p6j
From Proteopedia
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| - | [[ | + | ==Full-sequence computational design and solution structure of a thermostable protein variant== |
| + | <StructureSection load='2p6j' size='340' side='right' caption='[[2p6j]], [[NMR_Ensembles_of_Models | 43 NMR models]]' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[2p6j]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Unidentified Unidentified]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2P6J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2P6J FirstGlance]. <br> | ||
| + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2p6j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2p6j OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2p6j RCSB], [http://www.ebi.ac.uk/pdbsum/2p6j PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Computational protein design procedures were applied to the redesign of the entire sequence of a 51 amino acid residue protein, Drosophila melanogaster engrailed homeodomain. Various sequence optimization algorithms were compared and two resulting designed sequences were experimentally evaluated. The two sequences differ by 11 mutations and share 22% and 24% sequence identity with the wild-type protein. Both computationally designed proteins were considerably more stable than the naturally occurring protein, with midpoints of thermal denaturation greater than 99 degrees C. The solution structure was determined for one of the two sequences using multidimensional heteronuclear NMR spectroscopy, and the structure was found to closely match the original design template scaffold. | ||
| - | + | Full-sequence computational design and solution structure of a thermostable protein variant.,Shah PS, Hom GK, Ross SA, Lassila JK, Crowhurst KA, Mayo SL J Mol Biol. 2007 Sep 7;372(1):1-6. Epub 2007 Jun 16. PMID:17628593<ref>PMID:17628593</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | == References == | |
| - | + | <references/> | |
| - | == | + | __TOC__ |
| - | + | </StructureSection> | |
[[Category: Unidentified]] | [[Category: Unidentified]] | ||
[[Category: Crowhurst, K A.]] | [[Category: Crowhurst, K A.]] | ||
Revision as of 11:31, 20 October 2014
Full-sequence computational design and solution structure of a thermostable protein variant
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