1jtp

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[[Image:1jtp.gif|left|200px]]<br /><applet load="1jtp" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1jtp.gif|left|200px]]
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caption="1jtp, resolution 1.9&Aring;" />
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'''Degenerate interfaces in antigen-antibody complexes'''<br />
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{{Structure
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|PDB= 1jtp |SIZE=350|CAPTION= <scene name='initialview01'>1jtp</scene>, resolution 1.9&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=NA:SODIUM+ION'>NA</scene> and <scene name='pdbligand=FMT:FORMIC ACID'>FMT</scene>
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|ACTIVITY= [http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17]
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|GENE=
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}}
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'''Degenerate interfaces in antigen-antibody complexes'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1JTP is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Camelus_dromedarius Camelus dromedarius] and [http://en.wikipedia.org/wiki/Meleagris_gallopavo Meleagris gallopavo] with <scene name='pdbligand=NA:'>NA</scene> and <scene name='pdbligand=FMT:'>FMT</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JTP OCA].
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1JTP is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Camelus_dromedarius Camelus dromedarius] and [http://en.wikipedia.org/wiki/Meleagris_gallopavo Meleagris gallopavo]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JTP OCA].
==Reference==
==Reference==
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Degenerate interfaces in antigen-antibody complexes., Decanniere K, Transue TR, Desmyter A, Maes D, Muyldermans S, Wyns L, J Mol Biol. 2001 Oct 26;313(3):473-8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11676532 11676532]
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Degenerate interfaces in antigen-antibody complexes., Decanniere K, Transue TR, Desmyter A, Maes D, Muyldermans S, Wyns L, J Mol Biol. 2001 Oct 26;313(3):473-8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11676532 11676532]
[[Category: Camelus dromedarius]]
[[Category: Camelus dromedarius]]
[[Category: Lysozyme]]
[[Category: Lysozyme]]
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[[Category: vhh]]
[[Category: vhh]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:26:38 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:08:18 2008''

Revision as of 10:08, 20 March 2008

Image:1jtp.gif


PDB ID 1jtp

Drag the structure with the mouse to rotate
, resolution 1.9Å
Ligands: and
Activity: Lysozyme, with EC number 3.2.1.17
Coordinates: save as pdb, mmCIF, xml



Degenerate interfaces in antigen-antibody complexes


Overview

In most of the work dealing with the analysis of protein-protein interfaces, a single X-ray structure is available or selected, and implicitly it is assumed that this structure corresponds to the optimal complex for this pair of proteins. However, we have found a degenerate interface in a high-affinity antibody-antigen complex: the two independent complexes of the camel variable domain antibody fragment cAb-Lys3 and its antigen hen egg white lysozyme present in the asymmetric unit of our crystals show a difference in relative orientation between antibody and antigen, leading to important differences at the protein-protein interface. A third cAb-Lys3-hen lysozyme complex in a different crystal form adopts yet another relative orientation. Our results show that protein-protein interface characteristics can vary significantly between different specimens of the same high-affinity antibody-protein antigen complex. Consideration should be given to this type of observation when trying to establish general protein-protein interface characteristics.

About this Structure

1JTP is a Protein complex structure of sequences from Camelus dromedarius and Meleagris gallopavo. Full crystallographic information is available from OCA.

Reference

Degenerate interfaces in antigen-antibody complexes., Decanniere K, Transue TR, Desmyter A, Maes D, Muyldermans S, Wyns L, J Mol Biol. 2001 Oct 26;313(3):473-8. PMID:11676532

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