1jvu
From Proteopedia
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| - | [[Image:1jvu.gif|left|200px]] | + | [[Image:1jvu.gif|left|200px]] |
| - | + | ||
| - | '''CRYSTAL STRUCTURE OF RIBONUCLEASE A (COMPLEXED FORM)''' | + | {{Structure |
| + | |PDB= 1jvu |SIZE=350|CAPTION= <scene name='initialview01'>1jvu</scene>, resolution 1.78Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=C2P:CYTIDINE-2'-MONOPHOSPHATE'>C2P</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Pancreatic_ribonuclease Pancreatic ribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.5 3.1.27.5] | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''CRYSTAL STRUCTURE OF RIBONUCLEASE A (COMPLEXED FORM)''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1JVU is a [ | + | 1JVU is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JVU OCA]. |
==Reference== | ==Reference== | ||
| - | Reversible substrate-induced domain motions in ribonuclease A., Vitagliano L, Merlino A, Zagari A, Mazzarella L, Proteins. 2002 Jan 1;46(1):97-104. PMID:[http:// | + | Reversible substrate-induced domain motions in ribonuclease A., Vitagliano L, Merlino A, Zagari A, Mazzarella L, Proteins. 2002 Jan 1;46(1):97-104. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11746706 11746706] |
[[Category: Bos taurus]] | [[Category: Bos taurus]] | ||
[[Category: Pancreatic ribonuclease]] | [[Category: Pancreatic ribonuclease]] | ||
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[[Category: Zagari, A.]] | [[Category: Zagari, A.]] | ||
[[Category: C2P]] | [[Category: C2P]] | ||
| - | [[Category: protein | + | [[Category: protein dynamic]] |
[[Category: protein structure-function]] | [[Category: protein structure-function]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:09:13 2008'' |
Revision as of 10:09, 20 March 2008
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| , resolution 1.78Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Activity: | Pancreatic ribonuclease, with EC number 3.1.27.5 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
CRYSTAL STRUCTURE OF RIBONUCLEASE A (COMPLEXED FORM)
Overview
Despite the increasing number of successful determinations of complex protein structures the understanding of their dynamics properties is still rather limited. Using X-ray crystallography, we demonstrate that ribonuclease A (RNase A) undergoes significant domain motions upon ligand binding. In particular, when cytidine 2'-monophosphate binds to RNase A, the structure of the enzyme becomes more compact. Interestingly, our data also show that these structural alterations are fully reversible in the crystal state. These findings provide structural bases for the dynamic behavior of RNase A in the binding of the substrate shown by Petsko and coworkers (Rasmussen et al. Nature 1992;357:423-424). These subtle domain motions may assume functional relevance for more complex system and may play a significant role in the cooperativity of oligomeric enzymes.
About this Structure
1JVU is a Single protein structure of sequence from Bos taurus. Full crystallographic information is available from OCA.
Reference
Reversible substrate-induced domain motions in ribonuclease A., Vitagliano L, Merlino A, Zagari A, Mazzarella L, Proteins. 2002 Jan 1;46(1):97-104. PMID:11746706
Page seeded by OCA on Thu Mar 20 12:09:13 2008
