1jwx
From Proteopedia
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| - | [[Image:1jwx.jpg|left|200px]] | + | [[Image:1jwx.jpg|left|200px]] |
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| - | '''Chalcone Synthase--F215S mutant''' | + | {{Structure |
| + | |PDB= 1jwx |SIZE=350|CAPTION= <scene name='initialview01'>1jwx</scene>, resolution 1.63Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Naringenin-chalcone_synthase Naringenin-chalcone synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.74 2.3.1.74] | ||
| + | |GENE= CHS2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3879 Medicago sativa]) | ||
| + | }} | ||
| + | |||
| + | '''Chalcone Synthase--F215S mutant''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1JWX is a [ | + | 1JWX is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Medicago_sativa Medicago sativa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JWX OCA]. |
==Reference== | ==Reference== | ||
| - | Expanding the biosynthetic repertoire of plant type III polyketide synthases by altering starter molecule specificity., Jez JM, Bowman ME, Noel JP, Proc Natl Acad Sci U S A. 2002 Apr 16;99(8):5319-24. PMID:[http:// | + | Expanding the biosynthetic repertoire of plant type III polyketide synthases by altering starter molecule specificity., Jez JM, Bowman ME, Noel JP, Proc Natl Acad Sci U S A. 2002 Apr 16;99(8):5319-24. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11959984 11959984] |
[[Category: Medicago sativa]] | [[Category: Medicago sativa]] | ||
[[Category: Naringenin-chalcone synthase]] | [[Category: Naringenin-chalcone synthase]] | ||
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[[Category: polyketide synthase]] | [[Category: polyketide synthase]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:09:35 2008'' |
Revision as of 10:09, 20 March 2008
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| , resolution 1.63Å | |||||||
|---|---|---|---|---|---|---|---|
| Gene: | CHS2 (Medicago sativa) | ||||||
| Activity: | Naringenin-chalcone synthase, with EC number 2.3.1.74 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Chalcone Synthase--F215S mutant
Overview
Type III polyketide synthases (PKS) generate an array of natural products by condensing multiple acetyl units derived from malonyl-CoA to thioester-linked starter molecules covalently bound in the PKS active site. One strategy adopted by Nature for increasing the functional diversity of these biosynthetic enzymes involves modifying polyketide assembly by altering the preference for starter molecules. Chalcone synthase (CHS) is a ubiquitous plant PKS and the first type III PKS described functionally and structurally. Guided by the three-dimensional structure of CHS, Phe-215 and Phe-265, which are situated at the active site entrance, were targeted for site-directed mutagenesis to diversify CHS activity. The resulting mutants were screened against a panel of aliphatic and aromatic CoA-linked starter molecules to evaluate the degree of starter molecule specificity in CHS. Although wild-type CHS accepts a number of natural CoA thioesters, it does not use N-methylanthraniloyl-CoA as a substrate. Substitution of Phe-215 by serine yields a CHS mutant that preferentially accepts this CoA-thioester substrate to generate a novel alkaloid, namely N-methylanthraniloyltriacetic acid lactone. These results demonstrate that a point mutation in CHS dramatically shifts the molecular selectivity of this enzyme. This structure-based approach to metabolic redesign represents an initial step toward tailoring the biosynthetic activity of plant type III PKS.
About this Structure
1JWX is a Single protein structure of sequence from Medicago sativa. Full crystallographic information is available from OCA.
Reference
Expanding the biosynthetic repertoire of plant type III polyketide synthases by altering starter molecule specificity., Jez JM, Bowman ME, Noel JP, Proc Natl Acad Sci U S A. 2002 Apr 16;99(8):5319-24. PMID:11959984
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