1jxi
From Proteopedia
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| - | [[Image:1jxi.gif|left|200px]] | + | [[Image:1jxi.gif|left|200px]] |
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| - | '''4-Amino-5-hydroxymethyl-2-methylpyrimidine Phosphate Kinase from Salmonella typhimurium complexed with 4-Amino-5-hydroxymethyl-2-methylpyrimidine''' | + | {{Structure |
| + | |PDB= 1jxi |SIZE=350|CAPTION= <scene name='initialview01'>1jxi</scene>, resolution 2.64Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene> and <scene name='pdbligand=HMH:4-AMINO-5-HYDROXYMETHYL-2-METHYLPYRIMIDINE'>HMH</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Phosphomethylpyrimidine_kinase Phosphomethylpyrimidine kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.4.7 2.7.4.7] | ||
| + | |GENE= thiD ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=602 Salmonella typhimurium]) | ||
| + | }} | ||
| + | |||
| + | '''4-Amino-5-hydroxymethyl-2-methylpyrimidine Phosphate Kinase from Salmonella typhimurium complexed with 4-Amino-5-hydroxymethyl-2-methylpyrimidine''' | ||
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==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1JXI is a [ | + | 1JXI is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Salmonella_typhimurium Salmonella typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JXI OCA]. |
==Reference== | ==Reference== | ||
| - | Crystal structure of 4-amino-5-hydroxymethyl-2-methylpyrimidine phosphate kinase from Salmonella typhimurium at 2.3 A resolution., Cheng G, Bennett EM, Begley TP, Ealick SE, Structure. 2002 Feb;10(2):225-35. PMID:[http:// | + | Crystal structure of 4-amino-5-hydroxymethyl-2-methylpyrimidine phosphate kinase from Salmonella typhimurium at 2.3 A resolution., Cheng G, Bennett EM, Begley TP, Ealick SE, Structure. 2002 Feb;10(2):225-35. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11839308 11839308] |
[[Category: Phosphomethylpyrimidine kinase]] | [[Category: Phosphomethylpyrimidine kinase]] | ||
[[Category: Salmonella typhimurium]] | [[Category: Salmonella typhimurium]] | ||
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[[Category: thid]] | [[Category: thid]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:09:49 2008'' |
Revision as of 10:09, 20 March 2008
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| , resolution 2.64Å | |||||||
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| Ligands: | and | ||||||
| Gene: | thiD (Salmonella typhimurium) | ||||||
| Activity: | Phosphomethylpyrimidine kinase, with EC number 2.7.4.7 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
4-Amino-5-hydroxymethyl-2-methylpyrimidine Phosphate Kinase from Salmonella typhimurium complexed with 4-Amino-5-hydroxymethyl-2-methylpyrimidine
Overview
The crystal structures of Salmonella typhimurium 4-amino-5-hydroxymethyl-2-methylpyrimidine phosphate kinase (HMPP kinase) and its complex with substrate HMP have been determined. HMPP kinase catalyzes two separate ATP-dependent phosphorylation reactions and is an essential enzyme in the thiamin biosynthetic pathway. HMPP kinase is a homodimer with one active site per monomer and is structurally homologous to members of the ribokinase family. A comparison of the structure of HMPP kinase with other members of the ribokinase family suggests an evolutionary progression. Modeling studies suggest that HMPP kinase catalyzes both of its phosphorylation reactions using in-line displacement mechanisms. We propose that the active site accommodates the two separate reactions by providing two different binding modes for the phosphate group of HMP phosphate.
About this Structure
1JXI is a Single protein structure of sequence from Salmonella typhimurium. Full crystallographic information is available from OCA.
Reference
Crystal structure of 4-amino-5-hydroxymethyl-2-methylpyrimidine phosphate kinase from Salmonella typhimurium at 2.3 A resolution., Cheng G, Bennett EM, Begley TP, Ealick SE, Structure. 2002 Feb;10(2):225-35. PMID:11839308
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