2xdb

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[[Image:2xdb.png|left|200px]]
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==A PROCESSED NON-CODING RNA REGULATES A BACTERIAL ANTIVIRAL SYSTEM==
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<StructureSection load='2xdb' size='340' side='right' caption='[[2xdb]], [[Resolution|resolution]] 2.55&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2xdb]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Pectobacterium_atrosepticum Pectobacterium atrosepticum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XDB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2XDB FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=A23:ADENOSINE-5-PHOSPHATE-2,3-CYCLIC+PHOSPHATE'>A23</scene></td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2xd0|2xd0]], [[2xdd|2xdd]]</td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ribonuclease_T(1) Ribonuclease T(1)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.3 3.1.27.3] </span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2xdb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xdb OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2xdb RCSB], [http://www.ebi.ac.uk/pdbsum/2xdb PDBsum]</span></td></tr>
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The &gt;/=10(30) bacteriophages on Earth relentlessly drive adaptive coevolution, forcing the generation of protective mechanisms in their bacterial hosts. One such bacterial phage-resistance system, ToxIN, consists of a protein toxin (ToxN) that is inhibited in vivo by a specific RNA antitoxin (ToxI); however, the mechanisms for this toxicity and inhibition have not been defined. Here we present the crystal structure of the ToxN-ToxI complex from Pectobacterium atrosepticum, determined to 2.75-A resolution. ToxI is a 36-nucleotide noncoding RNA pseudoknot, and three ToxI monomers bind to three ToxN monomers to generate a trimeric ToxN-ToxI complex. Assembly of this complex is mediated entirely through extensive RNA-protein interactions. Furthermore, a 2'-3' cyclic phosphate at the 3' end of ToxI, and catalytic residues, identify ToxN as an endoRNase that processes ToxI from a repetitive precursor but is regulated by its own catalytic product.
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{{STRUCTURE_2xdb| PDB=2xdb | SCENE= }}
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A processed noncoding RNA regulates an altruistic bacterial antiviral system.,Blower TR, Pei XY, Short FL, Fineran PC, Humphreys DP, Luisi BF, Salmond GP Nat Struct Mol Biol. 2011 Feb;18(2):185-90. Epub 2011 Jan 16. PMID:21240270<ref>PMID:21240270</ref>
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===A PROCESSED NON-CODING RNA REGULATES A BACTERIAL ANTIVIRAL SYSTEM===
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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{{ABSTRACT_PUBMED_21240270}}
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== References ==
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<references/>
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==About this Structure==
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__TOC__
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[[2xdb]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Pectobacterium_atrosepticum Pectobacterium atrosepticum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XDB OCA].
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</StructureSection>
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==Reference==
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<ref group="xtra">PMID:021240270</ref><references group="xtra"/>
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[[Category: Pectobacterium atrosepticum]]
[[Category: Pectobacterium atrosepticum]]
[[Category: Blower, T R.]]
[[Category: Blower, T R.]]

Revision as of 13:05, 22 October 2014

A PROCESSED NON-CODING RNA REGULATES A BACTERIAL ANTIVIRAL SYSTEM

2xdb, resolution 2.55Å

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