2y10

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[[Image:2y10.png|left|200px]]
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==THE CRYSTAL STRUCTURE OF EF-TU AND TRP-TRNA-TRP BOUND TO A COGNATE CODON ON THE 70S RIBOSOME.==
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<StructureSection load='2y10' size='340' side='right' caption='[[2y10]], [[Resolution|resolution]] 3.10&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2y10]] is a 26 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Y10 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2Y10 FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=KIR:KIRROMYCIN'>KIR</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=4SU:4-THIOURIDINE-5-MONOPHOSPHATE'>4SU</scene>, <scene name='pdbligand=5MU:5-METHYLURIDINE+5-MONOPHOSPHATE'>5MU</scene>, <scene name='pdbligand=7MG:7N-METHYL-8-HYDROGUANOSINE-5-MONOPHOSPHATE'>7MG</scene>, <scene name='pdbligand=H2U:5,6-DIHYDROURIDINE-5-MONOPHOSPHATE'>H2U</scene>, <scene name='pdbligand=MIA:2-METHYLTHIO-N6-ISOPENTENYL-ADENOSINE-5-MONOPHOSPHATE'>MIA</scene>, <scene name='pdbligand=OMC:O2-METHYLYCYTIDINE-5-MONOPHOSPHATE'>OMC</scene>, <scene name='pdbligand=PSU:PSEUDOURIDINE-5-MONOPHOSPHATE'>PSU</scene></td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2y11|2y11]], [[2y18|2y18]], [[2y19|2y19]], [[1ibk|1ibk]], [[1ibl|1ibl]], [[2uuc|2uuc]], [[2wdk|2wdk]], [[2y0u|2y0u]], [[2j02|2j02]], [[1j5e|1j5e]], [[2wrq|2wrq]], [[2j00|2j00]], [[1hnx|1hnx]], [[2b9o|2b9o]], [[2x9t|2x9t]], [[2uub|2uub]], [[2wh3|2wh3]], [[1vov|1vov]], [[1emi|1emi]], [[2xqd|2xqd]], [[1pnx|1pnx]], [[2v46|2v46]], [[2vqf|2vqf]], [[2wh1|2wh1]], [[1n32|1n32]], [[2y12|2y12]], [[2wrn|2wrn]], [[2f4v|2f4v]], [[2jl5|2jl5]], [[1twt|1twt]], [[1qzc|1qzc]], [[2uxd|2uxd]], [[1fka|1fka]], [[1fjg|1fjg]], [[2uxc|2uxc]], [[1n36|1n36]], [[1hnw|1hnw]], [[1l1u|1l1u]], [[1hr0|1hr0]], [[2xuy|2xuy]], [[2v48|2v48]], [[2b64|2b64]], [[1xmq|1xmq]], [[2b9m|2b9m]], [[1pn8|1pn8]], [[2wdg|2wdg]], [[1xnr|1xnr]], [[1dv4|1dv4]], [[1hnz|1hnz]], [[1rss|1rss]], [[2wdh|2wdh]], [[1jgq|1jgq]], [[1i94|1i94]], [[2xsy|2xsy]], [[1i96|1i96]], [[2uu9|2uu9]], [[1pn7|1pn7]], [[1eg0|1eg0]], [[1ibm|1ibm]], [[2uxb|2uxb]], [[2vqe|2vqe]], [[1g1x|1g1x]], [[1ha3|1ha3]], [[2uua|2uua]], [[1i95|1i95]], [[2xfz|2xfz]], [[1xnq|1xnq]], [[1qd7|1qd7]], [[1gix|1gix]], [[1jgo|1jgo]], [[1jgp|1jgp]], [[2xg1|2xg1]], [[2c78|2c78]], [[2x9r|2x9r]], [[1n34|1n34]], [[1n33|1n33]], [[2y0w|2y0w]], [[2jl7|2jl7]], [[1pns|1pns]], [[1yl4|1yl4]], [[2y0y|2y0y]], [[2wdm|2wdm]], [[1xmo|1xmo]], [[1i97|1i97]], [[2y17|2y17]], [[2y15|2y15]], [[2y16|2y16]], [[2y14|2y14]]</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2y10 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2y10 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2y10 RCSB], [http://www.ebi.ac.uk/pdbsum/2y10 PDBsum]</span></td></tr>
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The ribosome converts genetic information into protein by selecting aminoacyl tRNAs whose anticodons base-pair to an mRNA codon. Mutations in the tRNA body can perturb this process and affect fidelity. The Hirsh suppressor is a well-studied tRNA(Trp) harboring a G24A mutation that allows readthrough of UGA stop codons. Here we present crystal structures of the 70S ribosome complexed with EF-Tu and aminoacyl tRNA (native tRNA(Trp), G24A tRNA(Trp) or the miscoding A9C tRNA(Trp)) bound to cognate UGG or near-cognate UGA codons, determined at 3.2-A resolution. The A9C and G24A mutations lead to miscoding by facilitating the distortion of tRNA required for decoding. A9C accomplishes this by increasing tRNA flexibility, whereas G24A allows the formation of an additional hydrogen bond that stabilizes the distortion. Our results also suggest that each native tRNA will adopt a unique conformation when delivered to the ribosome that allows accurate decoding.
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{{STRUCTURE_2y10| PDB=2y10 | SCENE= }}
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How mutations in tRNA distant from the anticodon affect the fidelity of decoding.,Schmeing TM, Voorhees RM, Kelley AC, Ramakrishnan V Nat Struct Mol Biol. 2011 Apr;18(4):432-6. Epub 2011 Mar 6. PMID:21378964<ref>PMID:21378964</ref>
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===THE CRYSTAL STRUCTURE OF EF-TU AND TRP-TRNA-TRP BOUND TO A COGNATE CODON ON THE 70S RIBOSOME.===
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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{{ABSTRACT_PUBMED_21378964}}
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==About this Structure==
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[[2y10]] is a 26 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Y10 OCA].
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==See Also==
==See Also==
*[[Elongation factor|Elongation factor]]
*[[Elongation factor|Elongation factor]]
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*[[Ribosomal protein S10|Ribosomal protein S10]]
 
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*[[Ribosomal protein S11|Ribosomal protein S11]]
 
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*[[Ribosomal protein S12|Ribosomal protein S12]]
 
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*[[Ribosomal protein S13|Ribosomal protein S13]]
 
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*[[Ribosomal protein S14|Ribosomal protein S14]]
 
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*[[Ribosomal protein S15|Ribosomal protein S15]]
 
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*[[Ribosomal protein S16|Ribosomal protein S16]]
 
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*[[Ribosomal protein S17|Ribosomal protein S17]]
 
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*[[Ribosomal protein S18|Ribosomal protein S18]]
 
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*[[Ribosomal protein S19|Ribosomal protein S19]]
 
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*[[Ribosomal protein S2|Ribosomal protein S2]]
 
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*[[Ribosomal protein S20|Ribosomal protein S20]]
 
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*[[Ribosomal protein S3|Ribosomal protein S3]]
 
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*[[Ribosomal protein S4|Ribosomal protein S4]]
 
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*[[Ribosomal protein S5|Ribosomal protein S5]]
 
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*[[Ribosomal protein S6|Ribosomal protein S6]]
 
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*[[Ribosomal protein S7|Ribosomal protein S7]]
 
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*[[Ribosomal protein S8|Ribosomal protein S8]]
 
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*[[Ribosomal protein S9|Ribosomal protein S9]]
 
*[[Ribosomal protein THX|Ribosomal protein THX]]
*[[Ribosomal protein THX|Ribosomal protein THX]]
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*[[Ribosome 3D structures|Ribosome 3D structures]]
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==Reference==
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== References ==
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<ref group="xtra">PMID:021378964</ref><references group="xtra"/>
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<references/>
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__TOC__
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</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
[[Category: Thermus thermophilus]]
[[Category: Thermus thermophilus]]

Revision as of 13:16, 22 October 2014

THE CRYSTAL STRUCTURE OF EF-TU AND TRP-TRNA-TRP BOUND TO A COGNATE CODON ON THE 70S RIBOSOME.

2y10, resolution 3.10Å

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