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3r0r
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| - | [[ | + | ==The 2.3 A structure of porcine circovirus 2== |
| + | <StructureSection load='3r0r' size='340' side='right' caption='[[3r0r]], [[Resolution|resolution]] 2.35Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[3r0r]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3R0R OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3R0R FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3r0r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3r0r OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3r0r RCSB], [http://www.ebi.ac.uk/pdbsum/3r0r PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Porcine circovirus 2 (PCV2) is a T=1 non-enveloped icosahedral virus that has had severe impact on the swine industry. Here we report the crystal structure of an N-terminally truncated PCV2 virus-like particle at 2.3 A resolution, and the cryo-EM image reconstruction of a full length PCV2 virus-like particle at 9.6 A resolution. This is the first atomic structure of a Circovirus. The crystal structure revealed that the capsid protein fold is a canonical viral jelly-roll. The loops connecting the strands of the jelly-roll define the limited features of the surface. Sulfate ions interacting with the surface, and electrostatic potential calculations strongly suggest a heparan sulfate binding site that allows PCV2 to gain entry into the cell. The crystal structure also allowed previously determined epitopes of the capsid to be visualized. The cryo-EM image reconstruction showed that the location of the N-terminus, absent in the crystal structure, is inside the capsid. As the N-terminus was previously shown to be antigenic it may externalize through viral 'breathing'. | ||
| - | + | The 2.3 A Structure of Porcine Circovirus 2.,Khayat R, Brunn N, Speir JA, Hardham JM, Ankenbauer RG, Schneemann A, Johnson JE J Virol. 2011 Jun 1. PMID:21632760<ref>PMID:21632760</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | == References == | |
| - | + | <references/> | |
| - | + | __TOC__ | |
| - | + | </StructureSection> | |
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[[Category: Johnson, J E.]] | [[Category: Johnson, J E.]] | ||
| - | [[Category: Khayat, R]] | + | [[Category: Khayat, R.]] |
[[Category: Speir, J A.]] | [[Category: Speir, J A.]] | ||
[[Category: Beta barrel]] | [[Category: Beta barrel]] | ||
Revision as of 09:07, 29 October 2014
The 2.3 A structure of porcine circovirus 2
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