1k5j
From Proteopedia
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| - | [[Image:1k5j.gif|left|200px]] | + | [[Image:1k5j.gif|left|200px]] |
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| - | '''The Crystal Structure of Nucleoplasmin-Core''' | + | {{Structure |
| + | |PDB= 1k5j |SIZE=350|CAPTION= <scene name='initialview01'>1k5j</scene>, resolution 2.30Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= Nucleoplasmin ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=8355 Xenopus laevis]) | ||
| + | }} | ||
| + | |||
| + | '''The Crystal Structure of Nucleoplasmin-Core''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1K5J is a [ | + | 1K5J is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Xenopus_laevis Xenopus laevis]. The following page contains interesting information on the relation of 1K5J with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb85_1.html Importins]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K5J OCA]. |
==Reference== | ==Reference== | ||
| - | The crystal structure of nucleoplasmin-core: implications for histone binding and nucleosome assembly., Dutta S, Akey IV, Dingwall C, Hartman KL, Laue T, Nolte RT, Head JF, Akey CW, Mol Cell. 2001 Oct;8(4):841-53. PMID:[http:// | + | The crystal structure of nucleoplasmin-core: implications for histone binding and nucleosome assembly., Dutta S, Akey IV, Dingwall C, Hartman KL, Laue T, Nolte RT, Head JF, Akey CW, Mol Cell. 2001 Oct;8(4):841-53. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11684019 11684019] |
[[Category: Importins]] | [[Category: Importins]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: pentamer]] | [[Category: pentamer]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:12:58 2008'' |
Revision as of 10:13, 20 March 2008
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| , resolution 2.30Å | |||||||
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| Gene: | Nucleoplasmin (Xenopus laevis) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
The Crystal Structure of Nucleoplasmin-Core
Overview
The efficient assembly of histone complexes and nucleosomes requires the participation of molecular chaperones. Currently, there is a paucity of data on their mechanism of action. We now present the structure of an N-terminal domain of nucleoplasmin (Np-core) at 2.3 A resolution. The Np-core monomer is an eight-stranded beta barrel that fits snugly within a stable pentamer. In the crystal, two pentamers associate to form a decamer. We show that both Np and Np-core are competent to assemble large complexes that contain the four core histones. Further experiments and modeling suggest that these complexes each contain five histone octamers which dock to a central Np decamer. This work has important ramifications for models of histone storage, sperm chromatin decondensation, and nucleosome assembly.
About this Structure
1K5J is a Single protein structure of sequence from Xenopus laevis. The following page contains interesting information on the relation of 1K5J with [Importins]. Full crystallographic information is available from OCA.
Reference
The crystal structure of nucleoplasmin-core: implications for histone binding and nucleosome assembly., Dutta S, Akey IV, Dingwall C, Hartman KL, Laue T, Nolte RT, Head JF, Akey CW, Mol Cell. 2001 Oct;8(4):841-53. PMID:11684019
Page seeded by OCA on Thu Mar 20 12:12:58 2008
Categories: Importins | Single protein | Xenopus laevis | Akey, C W. | Akey, I V. | Dingwall, C. | Dutta, S. | Hartman, K L. | Head, J F. | Laue, T. | Nolte, R T. | Beta-barrel | Beta-bulge | Jellyroll | Pentamer
