2yfv

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[[Image:2yfv.png|left|200px]]
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==THE HETEROTRIMERIC COMPLEX OF KLUYVEROMYCES LACTIS SCM3, CSE4 AND H4==
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<StructureSection load='2yfv' size='340' side='right' caption='[[2yfv]], [[Resolution|resolution]] 2.32&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2yfv]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Eukaryota Eukaryota]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YFV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2YFV FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene></td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2yfw|2yfw]]</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2yfv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2yfv OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2yfv RCSB], [http://www.ebi.ac.uk/pdbsum/2yfv PDBsum]</span></td></tr>
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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A specialized nucleosome is a component of all eukaryotic kinetochores. The core of this nucleosome contains a centromere-specific histone, CENP-A (the Cse4 gene product in budding yeast), instead of the usual H3. Assembly of a centromeric nucleosome depends on a specific chaperone, called Scm3 in yeast and HJURP in higher eukaryotes. We describe here the structure of a complex formed by an N-terminal fragment of Scm3 with the histone-fold domains of Cse4, and H4, all prepared as recombinant proteins derived from the budding yeast Kluyveromyces lactis. The contacts of Scm3 with Cse4 explain its selectivity for the centromere-specific histone; key residues at the interface are conserved in HJURP, indicating a common mechanism for centromeric-histone deposition. We also report the structure of a (Cse4 : H4)(2) heterotetramer; comparison with the structure of the Scm3:Cse4:H4 complex shows that tetramer formation and DNA-binding require displacement of Scm3 from the nucleosome core. The two structures together suggest that specific contacts between the chaperone and Cse4, rather than an altered overall structure of the nucleosome core, determine the selective presence of Cse4 at centromeres.
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{{STRUCTURE_2yfv| PDB=2yfv | SCENE= }}
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Recognition of the centromere-specific histone Cse4 by the chaperone Scm3.,Cho US, Harrison SC Proc Natl Acad Sci U S A. 2011 Jun 7;108(23):9367-71. Epub 2011 May 23. PMID:21606327<ref>PMID:21606327</ref>
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===THE HETEROTRIMERIC COMPLEX OF KLUYVEROMYCES LACTIS SCM3, CSE4 AND H4===
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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{{ABSTRACT_PUBMED_21606327}}
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== References ==
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<references/>
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==About this Structure==
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__TOC__
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[[2yfv]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Eukaryota Eukaryota]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YFV OCA].
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</StructureSection>
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==See Also==
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*[[Histone|Histone]]
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==Reference==
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<ref group="xtra">PMID:021606327</ref><references group="xtra"/>
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[[Category: Eukaryota]]
[[Category: Eukaryota]]
[[Category: Cho, U S.]]
[[Category: Cho, U S.]]

Revision as of 11:59, 29 October 2014

THE HETEROTRIMERIC COMPLEX OF KLUYVEROMYCES LACTIS SCM3, CSE4 AND H4

2yfv, resolution 2.32Å

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