1k9g
From Proteopedia
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| - | [[Image:1k9g.gif|left|200px]] | + | [[Image:1k9g.gif|left|200px]] |
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| - | '''Crystal Structure of the Complex of Cryptolepine-d(CCTAGG)2''' | + | {{Structure |
| + | |PDB= 1k9g |SIZE=350|CAPTION= <scene name='initialview01'>1k9g</scene>, resolution 1.4Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=DR1:5-METHYL-5H-INDOLO[3,2-B]QUINOLINE'>DR1</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''Crystal Structure of the Complex of Cryptolepine-d(CCTAGG)2''' | ||
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==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1K9G is a [ | + | 1K9G is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K9G OCA]. |
==Reference== | ==Reference== | ||
| - | The antimalarial and cytotoxic drug cryptolepine intercalates into DNA at cytosine-cytosine sites., Lisgarten JN, Coll M, Portugal J, Wright CW, Aymami J, Nat Struct Biol. 2002 Jan;9(1):57-60. PMID:[http:// | + | The antimalarial and cytotoxic drug cryptolepine intercalates into DNA at cytosine-cytosine sites., Lisgarten JN, Coll M, Portugal J, Wright CW, Aymami J, Nat Struct Biol. 2002 Jan;9(1):57-60. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11731803 11731803] |
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Aymami, J.]] | [[Category: Aymami, J.]] | ||
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[[Category: dna intercalator complex]] | [[Category: dna intercalator complex]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:14:19 2008'' |
Revision as of 10:14, 20 March 2008
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| , resolution 1.4Å | |||||||
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| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal Structure of the Complex of Cryptolepine-d(CCTAGG)2
Overview
Cryptolepine, a naturally occurring indoloquinoline alkaloid used as an antimalarial drug in Central and Western Africa, has been found to bind to DNA in a formerly unknown intercalation mode. Evidence from competition dialysis assays demonstrates that cryptolepine is able to bind CG-rich sequences containing nonalternating CC sites. Here we show that cryptolepine interacts with the CC sites of the DNA fragment d(CCTAGG)(2) in a base-stacking intercalation mode. This is the first DNA intercalator complex, from approximately 90 solved by X-ray crystallography, to bind a nonalternating (pyrimidine-pyrimidine) DNA sequence. The asymmetry of the drug induces a perfect stacking with the asymmetric site, allowing for the stability of the complex in the absence of hydrogen bonding interactions. The crystal structure of this antimalarial drug-DNA complex provides evidence for the first nonalternating intercalation and, as such, provides a basis for the design of new anticancer or antimalarial drugs.
About this Structure
1K9G is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
The antimalarial and cytotoxic drug cryptolepine intercalates into DNA at cytosine-cytosine sites., Lisgarten JN, Coll M, Portugal J, Wright CW, Aymami J, Nat Struct Biol. 2002 Jan;9(1):57-60. PMID:11731803
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