1k9y
From Proteopedia
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| - | [[Image:1k9y.gif|left|200px]] | + | [[Image:1k9y.gif|left|200px]] |
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| - | '''The PAPase Hal2p complexed with magnesium ions and reaction products: AMP and inorganic phosphate''' | + | {{Structure |
| + | |PDB= 1k9y |SIZE=350|CAPTION= <scene name='initialview01'>1k9y</scene>, resolution 1.90Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene> and <scene name='pdbligand=BME:BETA-MERCAPTOETHANOL'>BME</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/3'(2'),5'-bisphosphate_nucleotidase 3'(2'),5'-bisphosphate nucleotidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.7 3.1.3.7] | ||
| + | |GENE= HAL2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae]) | ||
| + | }} | ||
| + | |||
| + | '''The PAPase Hal2p complexed with magnesium ions and reaction products: AMP and inorganic phosphate''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1K9Y is a [ | + | 1K9Y is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K9Y OCA]. |
==Reference== | ==Reference== | ||
| - | Structural enzymology of Li(+)-sensitive/Mg(2+)-dependent phosphatases., Patel S, Martinez-Ripoll M, Blundell TL, Albert A, J Mol Biol. 2002 Jul 26;320(5):1087-94. PMID:[http:// | + | Structural enzymology of Li(+)-sensitive/Mg(2+)-dependent phosphatases., Patel S, Martinez-Ripoll M, Blundell TL, Albert A, J Mol Biol. 2002 Jul 26;320(5):1087-94. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12126627 12126627] |
[[Category: 3'(2'),5'-bisphosphate nucleotidase]] | [[Category: 3'(2'),5'-bisphosphate nucleotidase]] | ||
[[Category: Saccharomyces cerevisiae]] | [[Category: Saccharomyces cerevisiae]] | ||
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[[Category: salt tolerance]] | [[Category: salt tolerance]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:14:34 2008'' |
Revision as of 10:14, 20 March 2008
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| , resolution 1.90Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , and | ||||||
| Gene: | HAL2 (Saccharomyces cerevisiae) | ||||||
| Activity: | 3'(2'),5'-bisphosphate nucleotidase, with EC number 3.1.3.7 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
The PAPase Hal2p complexed with magnesium ions and reaction products: AMP and inorganic phosphate
Overview
Li(+)-sensitive/Mg(2+)-dependent phosphatases have attracted considerable attention since they have been proposed as targets for lithium therapy in the treatment of manic-depressive patients. The members of this enzyme superfamily display low levels of sequence identity while possessing a common fold and active site. Extensive structural and biochemical data demonstrate the direct involvement of two metal ions in catalysis, and show that lithium exerts its inhibitory action by blocking the products at the active site. By exploiting the different inhibitory properties of magnesium and calcium, we have been able to solve the X-ray structures of the Li(+)-sensitive/Mg(2+)-dependent 3'-phosphoadenosine-5'-phosphatase in complex with its substrate and with its products. The structural comparison of these complexes provides a 3D picture of the different stages of the catalytic cycle. This gives new insights into the understanding of the biological function of this group of enzymes and their lithium inhibition, and should assist in the design of improved inhibitors of therapeutic value.
About this Structure
1K9Y is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
Reference
Structural enzymology of Li(+)-sensitive/Mg(2+)-dependent phosphatases., Patel S, Martinez-Ripoll M, Blundell TL, Albert A, J Mol Biol. 2002 Jul 26;320(5):1087-94. PMID:12126627
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