3p62

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{{STRUCTURE_3p62| PDB=3p62 | SCENE= }}
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==Wild-type pentaerythritol tetranitrate reductase containing a C-terminal 8-histidine tag==
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===Wild-type pentaerythritol tetranitrate reductase containing a C-terminal 8-histidine tag===
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<StructureSection load='3p62' size='340' side='right' caption='[[3p62]], [[Resolution|resolution]] 1.40&Aring;' scene=''>
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{{ABSTRACT_PUBMED_21064170}}
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3p62]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterobacter_cloacae Enterobacter cloacae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3P62 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3P62 FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene></td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1h50|1h50]], [[3p67|3p67]]</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">onr, PETNR ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=550 Enterobacter cloacae])</td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/NADPH_dehydrogenase NADPH dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.6.99.1 1.6.99.1] </span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3p62 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3p62 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3p62 RCSB], [http://www.ebi.ac.uk/pdbsum/3p62 PDBsum]</span></td></tr>
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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This work describes the development of an automated robotic platform for the rapid screening of enzyme variants generated from directed evolution studies of pentraerythritol tetranitrate (PETN) reductase, a target for industrial biocatalysis. By using a 96-well format, near pure enzyme was recovered and was suitable for high throughput kinetic assays; this enabled rapid screening for improved and new activities from libraries of enzyme variants. Initial characterisation of several single site-saturation libraries targeted at active site residues of PETN reductase, are described. Two mutants (T26S and W102F) were shown to have switched in substrate enantiopreference against substrates (E)-2-aryl-1-nitropropene and alpha-methyl-trans-cinnamaldehyde, respectively, with an increase in ee (62 % (R) for W102F). In addition, the detection of mutants with weak activity against alpha,beta-unsaturated carboxylic acid substrates showed progress in the expansion of the substrate range of PETN reductase. These methods can readily be adapted for rapid evolution of enzyme variants with other oxidoreductase enzymes.
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==About this Structure==
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Focused directed evolution of pentaerythritol tetranitrate reductase by using automated anaerobic kinetic screening of site-saturated libraries.,Hulley ME, Toogood HS, Fryszkowska A, Mansell D, Stephens GM, Gardiner JM, Scrutton NS Chembiochem. 2010 Nov 22;11(17):2433-47. PMID:21064170<ref>PMID:21064170</ref>
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[[3p62]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterobacter_cloacae Enterobacter cloacae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3P62 OCA].
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
==See Also==
==See Also==
*[[Pentaerythritol tetranitrate reductase|Pentaerythritol tetranitrate reductase]]
*[[Pentaerythritol tetranitrate reductase|Pentaerythritol tetranitrate reductase]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:021064170</ref><references group="xtra"/>
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__TOC__
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</StructureSection>
[[Category: Enterobacter cloacae]]
[[Category: Enterobacter cloacae]]
[[Category: NADPH dehydrogenase]]
[[Category: NADPH dehydrogenase]]
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[[Category: Alpha]]
[[Category: Alpha]]
[[Category: Beta barrel]]
[[Category: Beta barrel]]
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[[Category: Old yellow enzyme family]]
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[[Category: Old Yellow Enzyme]]
[[Category: Oxidoreductase]]
[[Category: Oxidoreductase]]

Revision as of 07:58, 5 November 2014

Wild-type pentaerythritol tetranitrate reductase containing a C-terminal 8-histidine tag

3p62, resolution 1.40Å

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