1kbu
From Proteopedia
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| - | [[Image:1kbu.gif|left|200px]] | + | [[Image:1kbu.gif|left|200px]] |
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| - | '''CRE RECOMBINASE BOUND TO A LOXP HOLLIDAY JUNCTION''' | + | {{Structure |
| + | |PDB= 1kbu |SIZE=350|CAPTION= <scene name='initialview01'>1kbu</scene>, resolution 2.2Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= CRE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10711 Enterobacteria phage P21]) | ||
| + | }} | ||
| + | |||
| + | '''CRE RECOMBINASE BOUND TO A LOXP HOLLIDAY JUNCTION''' | ||
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==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1KBU is a [ | + | 1KBU is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Enterobacteria_phage_p21 Enterobacteria phage p21]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KBU OCA]. |
==Reference== | ==Reference== | ||
| - | The order of strand exchanges in Cre-LoxP recombination and its basis suggested by the crystal structure of a Cre-LoxP Holliday junction complex., Martin SS, Pulido E, Chu VC, Lechner TS, Baldwin EP, J Mol Biol. 2002 May 24;319(1):107-27. PMID:[http:// | + | The order of strand exchanges in Cre-LoxP recombination and its basis suggested by the crystal structure of a Cre-LoxP Holliday junction complex., Martin SS, Pulido E, Chu VC, Lechner TS, Baldwin EP, J Mol Biol. 2002 May 24;319(1):107-27. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12051940 12051940] |
[[Category: Enterobacteria phage p21]] | [[Category: Enterobacteria phage p21]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
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[[Category: site-specific recombinase]] | [[Category: site-specific recombinase]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:15:15 2008'' |
Revision as of 10:15, 20 March 2008
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| , resolution 2.2Å | |||||||
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| Gene: | CRE (Enterobacteria phage P21) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
CRE RECOMBINASE BOUND TO A LOXP HOLLIDAY JUNCTION
Overview
Cre recombinase uses two pairs of sequential cleavage and religation reactions to exchange homologous DNA strands between 34 base-pair (bp) LoxP recognition sequences. In the oligomeric recombination complex, a switch between "cleaving" and "non-cleaving" subunit conformations regulates the number, order, and regio-specificity of the strand exchanges. However, the particular sequence of events has been in question. From analysis of strand composition of the Holliday junction (HJ) intermediate, we determined that Cre initiates recombination of LoxP by cleaving the upper strand on the left arm. Cre preferred to react with the left arm of a LoxP suicide substrate, but at a similar rate to the right arm, indicating that the first strand to be exchanged is selected prior to cleavage. We propose that during complex assembly the cleaving subunit preferentially associates with the LoxP left arm, directing the first strand exchange to that side. In addition, this biased assembly would enforce productive orientation of LoxP sites in the recombination synapses. A novel Cre-HJ complex structure in which LoxP was oriented with the left arm bound by the cleaving Cre subunit suggested a physical basis for the strand exchange order. Lys86 and Lys201 interact with the left arm scissile adenine base differently than in structures that have a scissile guanine. These interactions are associated with positioning the 198-208 loop, a structural component of the conformational switch, in a configuration that is specific to the cleaving conformation. Our results suggest that strand exchange order and site alignment are regulated by an "induced fit" mechanism in which the cleaving conformation is selectively stabilized through protein-DNA interactions with the scissile base on the strand that is cleaved first.
About this Structure
1KBU is a Protein complex structure of sequences from Enterobacteria phage p21. Full crystallographic information is available from OCA.
Reference
The order of strand exchanges in Cre-LoxP recombination and its basis suggested by the crystal structure of a Cre-LoxP Holliday junction complex., Martin SS, Pulido E, Chu VC, Lechner TS, Baldwin EP, J Mol Biol. 2002 May 24;319(1):107-27. PMID:12051940
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