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2x3b

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[[Image:2x3b.png|left|200px]]
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==ASAP1 INACTIVE MUTANT E294A, AN EXTRACELLULAR TOXIC ZINC METALLOENDOPEPTIDASE==
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<StructureSection load='2x3b' size='340' side='right' caption='[[2x3b]], [[Resolution|resolution]] 2.28&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2x3b]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Aeromonas_salmonicida_subsp._achromogenes Aeromonas salmonicida subsp. achromogenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2X3B OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2X3B FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2x3c|2x3c]], [[2x3a|2x3a]]</td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Deuterolysin Deuterolysin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.39 3.4.24.39] </span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2x3b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2x3b OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2x3b RCSB], [http://www.ebi.ac.uk/pdbsum/2x3b PDBsum]</span></td></tr>
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Two mutants of the toxic extracellular zinc endopeptidase AsaP1 (AsaP1_E294Q and AsaP1_E294A) of Aeromonas salmonicida subsp. achromogenes were expressed in Escherichia coli and crystallized by the vapour-diffusion method. Crystals were obtained using several precipitants and different protein concentrations. Protein crystals were found in a monoclinic (C2) as well as an orthorhombic (P2(1)2(1)2(1)) space group. The crystals belonging to the monoclinic space group C2 had unit-cell parameters a = 103.4, b = 70.9, c = 54.9 A, beta = 109.3 degrees for AsaP1_E294A, and a = 98.5, b = 74.5, c = 54.7 A, beta = 112.4 degrees for AsaP1_E294Q. The unit-cell parameters of the orthorhombic crystal obtained for AsaP1_E294A were a = 57.9, b = 60.2, c = 183.6 A. The crystals of the two different mutants diffracted X-rays beyond 2.0 A resolution.
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{{STRUCTURE_2x3b| PDB=2x3b | SCENE= }}
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Crystallization and preliminary X-ray diffraction studies of AsaP1_E294A and AsaP1_E294Q, two inactive mutants of the toxic zinc metallopeptidase AsaP1 from Aeromonas salmonicida subsp. achromogenes.,Bogdanovic X, Singh RK, Hentschke J, Gudmundsdottir BK, Hinrichs W Acta Crystallogr Sect F Struct Biol Cryst Commun. 2009 Jul 1;65(Pt, 7):695-7. Epub 2009 Jun 27. PMID:19574642<ref>PMID:19574642</ref>
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===ASAP1 INACTIVE MUTANT E294A, AN EXTRACELLULAR TOXIC ZINC METALLOENDOPEPTIDASE===
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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{{ABSTRACT_PUBMED_19574642}}
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== References ==
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<references/>
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==About this Structure==
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__TOC__
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[[2x3b]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Aeromonas_salmonicida_subsp._achromogenes Aeromonas salmonicida subsp. achromogenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2X3B OCA].
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</StructureSection>
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==Reference==
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<ref group="xtra">PMID:019574642</ref><ref group="xtra">PMID:018952802</ref><references group="xtra"/>
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[[Category: Aeromonas salmonicida subsp. achromogenes]]
[[Category: Aeromonas salmonicida subsp. achromogenes]]
[[Category: Deuterolysin]]
[[Category: Deuterolysin]]

Revision as of 09:29, 5 November 2014

ASAP1 INACTIVE MUTANT E294A, AN EXTRACELLULAR TOXIC ZINC METALLOENDOPEPTIDASE

2x3b, resolution 2.28Å

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