VP24
From Proteopedia
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<StructureSection load='4d9o' size='340' side='right' caption='VP24 from the Reston Ebola Virus [[4d9o]]' scene=''> | <StructureSection load='4d9o' size='340' side='right' caption='VP24 from the Reston Ebola Virus [[4d9o]]' scene=''> | ||
== Introduction == | == Introduction == | ||
| - | VP24 is a protein present in the Ebola and Marburg viruses, both of which are members of ''Filoviridae'' family. Presently there are five strains of Ebola: Sudan, Reston, Zaire, Bundibugyo, and Taï Forest, each with minor differences in VP24 sequences <ref name=' | + | VP24 is a protein present in the Ebola and Marburg viruses, both of which are members of ''Filoviridae'' family. Presently there are five strains of Ebola: Sudan, Reston, Zaire, Bundibugyo, and Taï Forest, each with minor differences in VP24 sequences <ref name='Marburg '>pmid 24574400</ref>. |
== Function == | == Function == | ||
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== Structural Characteristics == | == Structural Characteristics == | ||
| - | The Ebola and Marburg VP24 proteins are 30% identical(1). They share a similar pyramidal shaped domain, as well as a few structures. Both viruses have two highly conserved pockets underneath the "pyramid's" base | + | The Ebola and Marburg VP24 proteins are 30% identical(1). They share a similar pyramidal shaped domain, as well as a few structures. Both viruses have two highly conserved pockets underneath the "pyramid's" base <ref name='Marburg '/>. Additionally, the N termini of Ebola (Zaire) and the Marburg virus are very similar in function. They are both used for oligomer and nucleocapsid formation <ref name='Marburg '/><ref>pmid 22371572</ref>. |
<scene name='60/602719/Chain_b/1'>Ebola Domain</scene> (Reston) | <scene name='60/602719/Chain_b/1'>Ebola Domain</scene> (Reston) | ||
| - | There are a few structural characteristics only found in the Ebola viruses. At the top of the pyramidal domain, there are α helices present which are thought to interact with the α karyopherin <ref name=' | + | There are a few structural characteristics only found in the Ebola viruses. At the top of the pyramidal domain, there are α helices present which are thought to interact with the α karyopherin <ref name='Marburg '/>. An α helix formed by the N-terminus runs from the top of the "pyramid" to another nearby VP24, where it binds to one of the pockets located underneath the "pyramid" <ref name='Marburg '/>. |
<scene name='60/602719/Marburg_vp24_domain/1'>Marburg Domain</scene> | <scene name='60/602719/Marburg_vp24_domain/1'>Marburg Domain</scene> | ||
| - | The Marburg domain has a beta shelf present that sticks out from the structure <ref name=' | + | The Marburg domain has a beta shelf present that sticks out from the structure <ref name='Marburg '/>. The Marburg VP24 doesn't use an alpha helix to bind to another VP24 like the Ebola VP24 <ref name='Marburg '/>. Instead, it uses a flexible strand that binds to a groove of a close-by VP24 <ref name='Marburg '/>. |
</StructureSection> | </StructureSection> | ||
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4. Edwards, R. M., Johnson, B., Mire, C.E., Xu, W., Shabman, R.S., Speller, L.N., Leung, D.W., Geisbert, T.W., Amarasinghe, G.K., Basler, C.F. The Marburg Virus VP24 Protein Interacts with Keap1 to Activate the Cytoprotective Antioxidant Response Pathway. Cell. 2014, Mar 27. 6 1017-1025 | 4. Edwards, R. M., Johnson, B., Mire, C.E., Xu, W., Shabman, R.S., Speller, L.N., Leung, D.W., Geisbert, T.W., Amarasinghe, G.K., Basler, C.F. The Marburg Virus VP24 Protein Interacts with Keap1 to Activate the Cytoprotective Antioxidant Response Pathway. Cell. 2014, Mar 27. 6 1017-1025 | ||
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| - | 5. Bharat, T.A.M., Noda, T., Riches, J.D., Kraehling, V., Kolesnikova, L., Becker, S., Kawaoka, Y., Briggs, J.A.G. Structural dissection of Ebola virus and its assembly determinants using cryo-electron tomography. PNAS. 2012, Feb 6 109(11) 4275-4280. | ||
<references/> | <references/> | ||
Revision as of 07:49, 6 November 2014
VP24
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References
- ↑ 1.0 1.1 1.2 1.3 1.4 1.5 1.6 1.7 Zhang AP, Bornholdt ZA, Abelson DM, Saphire EO. Crystal Structure of Marburg Virus VP24. J Virol. 2014 May;88(10):5859-63. doi: 10.1128/JVI.03565-13. Epub 2014 Feb 26. PMID:24574400 doi:http://dx.doi.org/10.1128/JVI.03565-13
- ↑ 2.0 2.1 Zhang AP, Bornholdt ZA, Abelson DM, Saphire EO. Crystal Structure of Marburg Virus VP24. J Virol. 2014 May;88(10):5859-63. doi: 10.1128/JVI.03565-13. Epub 2014 Feb 26. PMID:24574400 doi:http://dx.doi.org/10.1128/JVI.03565-13
- ↑ Bharat TA, Noda T, Riches JD, Kraehling V, Kolesnikova L, Becker S, Kawaoka Y, Briggs JA. Structural dissection of Ebola virus and its assembly determinants using cryo-electron tomography. Proc Natl Acad Sci U S A. 2012 Mar 13;109(11):4275-80. doi:, 10.1073/pnas.1120453109. Epub 2012 Feb 27. PMID:22371572 doi:http://dx.doi.org/10.1073/pnas.1120453109
3. Xu, W., Edwards, M.R., Borek, D.M., Feagins, A.R., Mittal, A., Alinger, J.B., Berry, K.N., Yen, B., Hamilton, J., Brett, T.J., Pappu, R.V., Leung, D.W., Basler, C.F., Amarasinghe, G.K. Ebola Virus VP24 Targets a Unique NLS Binding Site on Karyopherin Alpha 5 to Selectively Compete with Nuclear Import of Phosphorylated STAT1. Cell Host & Microbe. 2014, Aug 13;16 187-200
4. Edwards, R. M., Johnson, B., Mire, C.E., Xu, W., Shabman, R.S., Speller, L.N., Leung, D.W., Geisbert, T.W., Amarasinghe, G.K., Basler, C.F. The Marburg Virus VP24 Protein Interacts with Keap1 to Activate the Cytoprotective Antioxidant Response Pathway. Cell. 2014, Mar 27. 6 1017-1025
