1kft
From Proteopedia
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| - | [[Image:1kft.gif|left|200px]] | + | [[Image:1kft.gif|left|200px]] |
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| - | '''Solution Structure of the C-Terminal domain of UvrC from E-coli''' | + | {{Structure |
| + | |PDB= 1kft |SIZE=350|CAPTION= <scene name='initialview01'>1kft</scene> | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
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| + | '''Solution Structure of the C-Terminal domain of UvrC from E-coli''' | ||
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==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1KFT is a [ | + | 1KFT is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KFT OCA]. |
==Reference== | ==Reference== | ||
| - | Solution structure and DNA-binding properties of the C-terminal domain of UvrC from E.coli., Singh S, Folkers GE, Bonvin AM, Boelens R, Wechselberger R, Niztayev A, Kaptein R, EMBO J. 2002 Nov 15;21(22):6257-66. PMID:[http:// | + | Solution structure and DNA-binding properties of the C-terminal domain of UvrC from E.coli., Singh S, Folkers GE, Bonvin AM, Boelens R, Wechselberger R, Niztayev A, Kaptein R, EMBO J. 2002 Nov 15;21(22):6257-66. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12426397 12426397] |
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: hhh domain]] | [[Category: hhh domain]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:16:55 2008'' |
Revision as of 10:16, 20 March 2008
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Solution Structure of the C-Terminal domain of UvrC from E-coli
Overview
The C-terminal domain of the UvrC protein (UvrC CTD) is essential for 5' incision in the prokaryotic nucleotide excision repair process. We have determined the three-dimensional structure of the UvrC CTD using heteronuclear NMR techniques. The structure shows two helix-hairpin-helix (HhH) motifs connected by a small connector helix. The UvrC CTD is shown to mediate structure-specific DNA binding. The domain binds to a single-stranded-double-stranded junction DNA, with a strong specificity towards looped duplex DNA that contains at least six unpaired bases per loop ("bubble DNA"). Using chemical shift perturbation experiments, the DNA-binding surface is mapped to the first hairpin region encompassing the conserved glycine-valine-glycine residues followed by lysine-arginine-arginine, a positively charged surface patch and the second hairpin region consisting of glycine-isoleucine-serine. A model for the protein-DNA complex is proposed that accounts for this specificity.
About this Structure
1KFT is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Solution structure and DNA-binding properties of the C-terminal domain of UvrC from E.coli., Singh S, Folkers GE, Bonvin AM, Boelens R, Wechselberger R, Niztayev A, Kaptein R, EMBO J. 2002 Nov 15;21(22):6257-66. PMID:12426397
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