1kls

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[[Image:1kls.jpg|left|200px]]<br /><applet load="1kls" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1kls.jpg|left|200px]]
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caption="1kls" />
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'''NMR Structure of the ZFY-6T[Y10L] Zinc Finger'''<br />
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{{Structure
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|PDB= 1kls |SIZE=350|CAPTION= <scene name='initialview01'>1kls</scene>
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|SITE=
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|LIGAND= <scene name='pdbligand=ZN:ZINC ION'>ZN</scene>
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|ACTIVITY=
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|GENE=
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}}
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'''NMR Structure of the ZFY-6T[Y10L] Zinc Finger'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1KLS is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=ZN:'>ZN</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KLS OCA].
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1KLS is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KLS OCA].
==Reference==
==Reference==
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The hidden thermodynamics of a zinc finger., Lachenmann MJ, Ladbury JE, Phillips NB, Narayana N, Qian X, Weiss MA, J Mol Biol. 2002 Mar 1;316(4):969-89. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11884136 11884136]
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The hidden thermodynamics of a zinc finger., Lachenmann MJ, Ladbury JE, Phillips NB, Narayana N, Qian X, Weiss MA, J Mol Biol. 2002 Mar 1;316(4):969-89. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11884136 11884136]
[[Category: Single protein]]
[[Category: Single protein]]
[[Category: Lachenmann, M J.]]
[[Category: Lachenmann, M J.]]
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[[Category: zinc finger]]
[[Category: zinc finger]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:35:29 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:19:07 2008''

Revision as of 10:19, 20 March 2008


PDB ID 1kls

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NMR Structure of the ZFY-6T[Y10L] Zinc Finger


Contents

Overview

The Zn finger provides a model for studies of protein structure and stability. Its core contains a conserved phenylalanine residue adjoining three architectural elements: a beta-hairpin, an alpha-helix and a tetrahedral Zn(2+)-binding site. Here, we demonstrate that the consensus Phe is not required for high-affinity Zn(2+) binding but contributes to the specification of a precise DNA-binding surface. Substitution of Phe by leucine in a ZFY peptide permits Zn(2+)-dependent folding. Although a native-like structure is retained, structural fluctuations lead to attenuation of selected nuclear Overhauser enhancements and accelerated amide proton exchange. Surprisingly, wild-type Zn affinity is maintained by entropy-enthalpy compensation (EEC): a hidden entropy penalty (TDeltaDeltaS 7kcal/mol) is balanced by enhanced enthalpy of association (DeltaDeltaH -7kcal/mol) at 25 degrees C. Because the variant is less well ordered than the Phe-anchored domain, the net change in entropy is opposite to the apparent change in configurational entropy. By analogy to the thermodynamics of organometallic complexation, we propose that EEC arises from differences in solvent reorganization. Exclusion of Leu among biological sequences suggests an evolutionary constraint on the dynamics of a Zn finger.

Disease

Known disease associated with this structure: Spastic paraplegia 33 OMIM:[610243]

About this Structure

1KLS is a Single protein structure of sequence from [1]. Full crystallographic information is available from OCA.

Reference

The hidden thermodynamics of a zinc finger., Lachenmann MJ, Ladbury JE, Phillips NB, Narayana N, Qian X, Weiss MA, J Mol Biol. 2002 Mar 1;316(4):969-89. PMID:11884136

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