1kn1
From Proteopedia
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| - | [[Image:1kn1.gif|left|200px]] | + | [[Image:1kn1.gif|left|200px]] |
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| - | '''Crystal structure of allophycocyanin''' | + | {{Structure |
| + | |PDB= 1kn1 |SIZE=350|CAPTION= <scene name='initialview01'>1kn1</scene>, resolution 2.2Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=BLA:BILIVERDINE+IX+ALPHA'>BLA</scene> and <scene name='pdbligand=CYC:PHYCOCYANOBILIN'>CYC</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''Crystal structure of allophycocyanin''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1KN1 is a [ | + | 1KN1 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Porphyra_yezoensis Porphyra yezoensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KN1 OCA]. |
==Reference== | ==Reference== | ||
| - | Crystal structure of allophycocyanin from red algae Porphyra yezoensis at 2.2-A resolution., Liu JY, Jiang T, Zhang JP, Liang DC, J Biol Chem. 1999 Jun 11;274(24):16945-52. PMID:[http:// | + | Crystal structure of allophycocyanin from red algae Porphyra yezoensis at 2.2-A resolution., Liu JY, Jiang T, Zhang JP, Liang DC, J Biol Chem. 1999 Jun 11;274(24):16945-52. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10358042 10358042] |
[[Category: Porphyra yezoensis]] | [[Category: Porphyra yezoensis]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
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[[Category: helix-turn-helix]] | [[Category: helix-turn-helix]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:19:34 2008'' |
Revision as of 10:19, 20 March 2008
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| , resolution 2.2Å | |||||||
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| Ligands: | and | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of allophycocyanin
Overview
The crystal structure of allophycocyanin from red algae Porphyra yezoensis (APC-PY) at 2.2-A resolution has been determined by the molecular replacement method. The crystal belongs to space group R32 with cell parameters a = b = 105.3 A, c = 189.4 A, alpha = beta = 90 degrees, gamma = 120 degrees. After several cycles of refinement using program X-PLOR and model building based on the electron density map, the crystallographic R-factor converged to 19.3% (R-free factor is 26.9%) in the range of 10.0 to 2.2 A. The r.m.s. deviations of bond length and angles are 0.015 A and 2.9 degrees, respectively. In the crystal, two APC-PY trimers associate face to face into a hexamer. The assembly of two trimers within the hexamer is similar to that of C-phycocyanin (C-PC) and R-phycoerythrin (R-PE) hexamers, but the assembly tightness of the two trimers to the hexamer is not so high as that in C-PC and R-PE hexamers. The chromophore-protein interactions and possible pathway of energy transfer were discussed. Phycocyanobilin 1alpha84 of APC-PY forms 5 hydrogen bonds with 3 residues in subunit 2beta of another monomer. In R-PE and C-PC, chromophore 1alpha84 only forms 1 hydrogen bond with 2beta77 residue in subunit 2beta. This result may support and explain great spectrum difference exists between APC trimer and monomer.
About this Structure
1KN1 is a Protein complex structure of sequences from Porphyra yezoensis. Full crystallographic information is available from OCA.
Reference
Crystal structure of allophycocyanin from red algae Porphyra yezoensis at 2.2-A resolution., Liu JY, Jiang T, Zhang JP, Liang DC, J Biol Chem. 1999 Jun 11;274(24):16945-52. PMID:10358042
Page seeded by OCA on Thu Mar 20 12:19:34 2008
Categories: Porphyra yezoensis | Protein complex | Chang, W R. | Jiang, T. | Liang, D C. | Liu, J Y. | Zhang, J P. | BLA | CYC | Helix-turn-helix
