1kp8
From Proteopedia
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| - | [[Image:1kp8.gif|left|200px]] | + | [[Image:1kp8.gif|left|200px]] |
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| - | '''Structural Basis for GroEL-assisted Protein Folding from the Crystal Structure of (GroEL-KMgATP)14 at 2.0 A Resolution''' | + | {{Structure |
| + | |PDB= 1kp8 |SIZE=350|CAPTION= <scene name='initialview01'>1kp8</scene>, resolution 2.00Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene> and <scene name='pdbligand=ATP:ADENOSINE-5'-TRIPHOSPHATE'>ATP</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''Structural Basis for GroEL-assisted Protein Folding from the Crystal Structure of (GroEL-KMgATP)14 at 2.0 A Resolution''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1KP8 is a [ | + | 1KP8 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. This structure supersedes the now removed PDB entry 1DER. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KP8 OCA]. |
==Reference== | ==Reference== | ||
| - | Structural basis for GroEL-assisted protein folding from the crystal structure of (GroEL-KMgATP)14 at 2.0A resolution., Wang J, Boisvert DC, J Mol Biol. 2003 Apr 4;327(4):843-55. PMID:[http:// | + | Structural basis for GroEL-assisted protein folding from the crystal structure of (GroEL-KMgATP)14 at 2.0A resolution., Wang J, Boisvert DC, J Mol Biol. 2003 Apr 4;327(4):843-55. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12654267 12654267] |
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: groel]] | [[Category: groel]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:20:23 2008'' |
Revision as of 10:20, 20 March 2008
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| , resolution 2.00Å | |||||||
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| Ligands: | , , and | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Structural Basis for GroEL-assisted Protein Folding from the Crystal Structure of (GroEL-KMgATP)14 at 2.0 A Resolution
Overview
Nucleotide regulates the affinity of the bacterial chaperonin GroEL for protein substrates. GroEL binds protein substrates with high affinity in the absence of ATP and with low affinity in its presence. We report the crystal structure of (GroEL-KMgATP)(14) refined to 2.0 A resolution in which the ATP triphosphate moiety is directly coordinated by both K(+) and Mg(2+). Upon the binding of KMgATP, we observe previously unnoticed domain rotations and a 102 degrees rotation of the apical domain surface helix I. Two major consequences are a large lateral displacement of, and a dramatic reduction of hydrophobicity in, the apical domain surface. These results provide a basis for the nucleotide-dependent regulation of protein substrate binding and suggest a mechanism for GroEL-assisted protein folding by forced unfolding.
About this Structure
1KP8 is a Single protein structure of sequence from Escherichia coli. This structure supersedes the now removed PDB entry 1DER. Full crystallographic information is available from OCA.
Reference
Structural basis for GroEL-assisted protein folding from the crystal structure of (GroEL-KMgATP)14 at 2.0A resolution., Wang J, Boisvert DC, J Mol Biol. 2003 Apr 4;327(4):843-55. PMID:12654267
Page seeded by OCA on Thu Mar 20 12:20:23 2008
Categories: Escherichia coli | Single protein | Wang, J. | ATP | K | MG | SO4 | Assisted protein folding | Chaperonin | Groel
