1kqs

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[[Image:1kqs.gif|left|200px]]<br /><applet load="1kqs" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1kqs.gif|left|200px]]
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caption="1kqs, resolution 3.10&Aring;" />
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'''The Haloarcula marismortui 50S Complexed with a Pretranslocational Intermediate in Protein Synthesis'''<br />
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{{Structure
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|PDB= 1kqs |SIZE=350|CAPTION= <scene name='initialview01'>1kqs</scene>, resolution 3.10&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=CD:CADMIUM+ION'>CD</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=PPU:PUROMYCIN-5'-MONOPHOSPHATE'>PPU</scene>, <scene name='pdbligand=PHA:PHENYLALANINAL'>PHA</scene>, <scene name='pdbligand=ACA:6-AMINOHEXANOIC+ACID'>ACA</scene> and <scene name='pdbligand=BTN:BIOTIN'>BTN</scene>
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|ACTIVITY=
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|GENE=
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}}
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'''The Haloarcula marismortui 50S Complexed with a Pretranslocational Intermediate in Protein Synthesis'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1KQS is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Haloarcula_marismortui Haloarcula marismortui] with <scene name='pdbligand=MG:'>MG</scene>, <scene name='pdbligand=K:'>K</scene>, <scene name='pdbligand=NA:'>NA</scene>, <scene name='pdbligand=CD:'>CD</scene>, <scene name='pdbligand=CL:'>CL</scene>, <scene name='pdbligand=PPU:'>PPU</scene>, <scene name='pdbligand=PHA:'>PHA</scene>, <scene name='pdbligand=ACA:'>ACA</scene> and <scene name='pdbligand=BTN:'>BTN</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KQS OCA].
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1KQS is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Haloarcula_marismortui Haloarcula marismortui]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KQS OCA].
==Reference==
==Reference==
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A pre-translocational intermediate in protein synthesis observed in crystals of enzymatically active 50S subunits., Schmeing TM, Seila AC, Hansen JL, Freeborn B, Soukup JK, Scaringe SA, Strobel SA, Moore PB, Steitz TA, Nat Struct Biol. 2002 Mar;9(3):225-30. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11828326 11828326]
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A pre-translocational intermediate in protein synthesis observed in crystals of enzymatically active 50S subunits., Schmeing TM, Seila AC, Hansen JL, Freeborn B, Soukup JK, Scaringe SA, Strobel SA, Moore PB, Steitz TA, Nat Struct Biol. 2002 Mar;9(3):225-30. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11828326 11828326]
[[Category: Haloarcula marismortui]]
[[Category: Haloarcula marismortui]]
[[Category: Protein complex]]
[[Category: Protein complex]]
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[[Category: rna-rna]]
[[Category: rna-rna]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:36:57 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:21:01 2008''

Revision as of 10:21, 20 March 2008

Image:1kqs.gif


PDB ID 1kqs

Drag the structure with the mouse to rotate
, resolution 3.10Å
Ligands: , , , , , , , and
Coordinates: save as pdb, mmCIF, xml



The Haloarcula marismortui 50S Complexed with a Pretranslocational Intermediate in Protein Synthesis


Overview

The large ribosomal subunit catalyzes peptide bond formation during protein synthesis. Its peptidyl transferase activity has often been studied using a 'fragment assay' that depends on high concentrations of methanol or ethanol. Here we describe a version of this assay that does not require alcohol and use it to show, both crystallographically and biochemically, that crystals of the large ribosomal subunits from Haloarcula marismortui are enzymatically active. Addition of these crystals to solutions containing substrates results in formation of products, which ceases when crystals are removed. When substrates are diffused into large subunit crystals, the subsequent structure shows that products have formed. The CC-puromycin-peptide product is found bound to the A-site and the deacylated CCA is bound to the P-site, with its 3prime prime or minute OH near N3 A2486 (Escherichia coli A2451). Thus, this structure represents a state that occurs after peptide bond formation but before the hybrid state of protein synthesis.

About this Structure

1KQS is a Protein complex structure of sequences from Haloarcula marismortui. Full crystallographic information is available from OCA.

Reference

A pre-translocational intermediate in protein synthesis observed in crystals of enzymatically active 50S subunits., Schmeing TM, Seila AC, Hansen JL, Freeborn B, Soukup JK, Scaringe SA, Strobel SA, Moore PB, Steitz TA, Nat Struct Biol. 2002 Mar;9(3):225-30. PMID:11828326

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