1ksw

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[[Image:1ksw.gif|left|200px]]<br /><applet load="1ksw" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1ksw.gif|left|200px]]
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caption="1ksw, resolution 2.8&Aring;" />
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'''Structure of Human c-Src Tyrosine Kinase (Thr338Gly Mutant) in Complex with N6-benzyl ADP'''<br />
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{{Structure
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|PDB= 1ksw |SIZE=350|CAPTION= <scene name='initialview01'>1ksw</scene>, resolution 2.8&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=NBS:N6-BENZYL ADENOSINE-5'-DIPHOSPHATE'>NBS</scene>
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|ACTIVITY= [http://en.wikipedia.org/wiki/Transferase Transferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.10.1 and 2.7.10.2 2.7.10.1 and 2.7.10.2]
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|GENE=
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}}
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'''Structure of Human c-Src Tyrosine Kinase (Thr338Gly Mutant) in Complex with N6-benzyl ADP'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1KSW is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=NBS:'>NBS</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Transferase Transferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.10.1 and 2.7.10.2 2.7.10.1 and 2.7.10.2] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KSW OCA].
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1KSW is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KSW OCA].
==Reference==
==Reference==
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Mutant tyrosine kinases with unnatural nucleotide specificity retain the structure and phospho-acceptor specificity of the wild-type enzyme., Witucki LA, Huang X, Shah K, Liu Y, Kyin S, Eck MJ, Shokat KM, Chem Biol. 2002 Jan;9(1):25-33. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11841936 11841936]
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Mutant tyrosine kinases with unnatural nucleotide specificity retain the structure and phospho-acceptor specificity of the wild-type enzyme., Witucki LA, Huang X, Shah K, Liu Y, Kyin S, Eck MJ, Shokat KM, Chem Biol. 2002 Jan;9(1):25-33. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11841936 11841936]
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: bump hole]]
[[Category: bump hole]]
[[Category: bump-hole]]
[[Category: bump-hole]]
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[[Category: chemical genetics]]
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[[Category: chemical genetic]]
[[Category: kinase]]
[[Category: kinase]]
[[Category: orthogonal substrate]]
[[Category: orthogonal substrate]]
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[[Category: sh3]]
[[Category: sh3]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:37:33 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:21:45 2008''

Revision as of 10:21, 20 March 2008

Image:1ksw.gif


PDB ID 1ksw

Drag the structure with the mouse to rotate
, resolution 2.8Å
Ligands:
Activity: Transferase, with EC number and 2.7.10.2 2.7.10.1 and 2.7.10.2
Coordinates: save as pdb, mmCIF, xml



Structure of Human c-Src Tyrosine Kinase (Thr338Gly Mutant) in Complex with N6-benzyl ADP


Contents

Overview

The direct substrates of one protein kinase in a cell can be identified by mutation of the ATP binding pocket to allow an unnatural ATP analog to be accepted exclusively by the engineered kinase. Here, we present structural and functional assessment of peptide specificity of mutant protein kinases with unnatural ATP analogs. The crystal structure (2.8 A resolution) of c-Src (T338G) with N(6)-(benzyl) ADP bound shows that the creation of a unique nucleotide binding pocket does not alter the phospho-acceptor binding site of the kinase. A panel of optimal peptide substrates of defined sequence, as well as a degenerate peptide library, was utilized to assess the phospho-acceptor specificity of the engineered "traceable" kinases. The specificity profiles for the mutant kinases were found to be identical to those of their wild-type counterparts.

Disease

Known disease associated with this structure: Colon cancer, advanced OMIM:[190090]

About this Structure

1KSW is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Mutant tyrosine kinases with unnatural nucleotide specificity retain the structure and phospho-acceptor specificity of the wild-type enzyme., Witucki LA, Huang X, Shah K, Liu Y, Kyin S, Eck MJ, Shokat KM, Chem Biol. 2002 Jan;9(1):25-33. PMID:11841936

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