1kxk
From Proteopedia
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| - | [[Image:1kxk.gif|left|200px]] | + | [[Image:1kxk.gif|left|200px]] |
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| - | '''Crystal Structure of a RNA Molecule Containing Domain 5 and 6 of the Yeast ai5g Group II Self-splicing Intron''' | + | {{Structure |
| + | |PDB= 1kxk |SIZE=350|CAPTION= <scene name='initialview01'>1kxk</scene>, resolution 3.0Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=MG:MAGNESIUM ION'>MG</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''Crystal Structure of a RNA Molecule Containing Domain 5 and 6 of the Yeast ai5g Group II Self-splicing Intron''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1KXK is a [ | + | 1KXK is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KXK OCA]. |
==Reference== | ==Reference== | ||
| - | Structural insights into group II intron catalysis and branch-site selection., Zhang L, Doudna JA, Science. 2002 Mar 15;295(5562):2084-8. Epub 2002 Feb 21. PMID:[http:// | + | Structural insights into group II intron catalysis and branch-site selection., Zhang L, Doudna JA, Science. 2002 Mar 15;295(5562):2084-8. Epub 2002 Feb 21. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11859154 11859154] |
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Doudna, J A.]] | [[Category: Doudna, J A.]] | ||
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[[Category: two-nucleotide bulge]] | [[Category: two-nucleotide bulge]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:23:32 2008'' |
Revision as of 10:23, 20 March 2008
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| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal Structure of a RNA Molecule Containing Domain 5 and 6 of the Yeast ai5g Group II Self-splicing Intron
Overview
Group II self-splicing introns catalyze autoexcision from precursor RNA transcripts by a mechanism strikingly similar to that of the spliceosome, an RNA-protein assembly responsible for splicing together the protein-coding parts of most eukaryotic pre-mRNAs. Splicing in both cases initiates via nucleophilic attack at the 5' splice site by the 2' OH of a conserved intron adenosine residue, creating a branched (lariat) intermediate. Here, we describe the crystal structure at 3.0 A resolution of a 70-nucleotide RNA containing the catalytically essential domains 5 and 6 of the yeast ai5gamma group II self-splicing intron, revealing an unexpected two-nucleotide bulged structure around the branch-point adenosine in domain 6.
About this Structure
1KXK is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
Structural insights into group II intron catalysis and branch-site selection., Zhang L, Doudna JA, Science. 2002 Mar 15;295(5562):2084-8. Epub 2002 Feb 21. PMID:11859154
Page seeded by OCA on Thu Mar 20 12:23:32 2008
