2yii
From Proteopedia
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| - | [[ | + | ==Manipulating the regioselectivity of phenylalanine aminomutase: new insights into the reaction mechanism of MIO-dependent enzymes from structure-guided directed evolution== |
| + | <StructureSection load='2yii' size='340' side='right' caption='[[2yii]], [[Resolution|resolution]] 2.18Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[2yii]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Taxus_wallichiana_var._chinensis Taxus wallichiana var. chinensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YII OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2YII FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BME:BETA-MERCAPTOETHANOL'>BME</scene>, <scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> | ||
| + | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MDO:{2-[(1S)-1-AMINOETHYL]-4-METHYLIDENE-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>MDO</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2yii FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2yii OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2yii RCSB], [http://www.ebi.ac.uk/pdbsum/2yii PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Turn to switch: A mutant of phenylalanine aminomutase was engineered that can catalyze the regioselective amination of cinnamate derivatives (see scheme, red) to, for example, beta-amino acids. This regioselectivity, along with the X-ray crystal structures, suggests two distinct carboxylate binding modes differentiated by C(beta)-C(ipso) bond rotation, which determines if beta- (see scheme) or alpha-addition takes place. | ||
| - | + | Mechanism-inspired engineering of phenylalanine aminomutase for enhanced beta-regioselective asymmetric amination of cinnamates.,Wu B, Szymanski W, Wybenga GG, Heberling MM, Bartsch S, de Wildeman S, Poelarends GJ, Feringa BL, Dijkstra BW, Janssen DB Angew Chem Int Ed Engl. 2012 Jan 9;51(2):482-6. doi: 10.1002/anie.201106372. Epub, 2011 Nov 23. PMID:22113970<ref>PMID:22113970</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | == References == | |
| - | + | <references/> | |
| - | == | + | __TOC__ |
| - | + | </StructureSection> | |
[[Category: Taxus wallichiana var. chinensis]] | [[Category: Taxus wallichiana var. chinensis]] | ||
| - | [[Category: Bartsch, S | + | [[Category: Bartsch, S]] |
| - | [[Category: Dijkstra, B W | + | [[Category: Dijkstra, B W]] |
| - | [[Category: Feringa, B L | + | [[Category: Feringa, B L]] |
| - | [[Category: Heberling, M M | + | [[Category: Heberling, M M]] |
| - | [[Category: Janssen, D B | + | [[Category: Janssen, D B]] |
| - | [[Category: Poelarends, G J | + | [[Category: Poelarends, G J]] |
| - | [[Category: Szymanski, W | + | [[Category: Szymanski, W]] |
| - | [[Category: Wildeman, S | + | [[Category: Wildeman, S]] |
| - | [[Category: Wu, B | + | [[Category: Wu, B]] |
| - | [[Category: Wybenga, G G | + | [[Category: Wybenga, G G]] |
[[Category: Lyase]] | [[Category: Lyase]] | ||
Revision as of 07:56, 12 November 2014
Manipulating the regioselectivity of phenylalanine aminomutase: new insights into the reaction mechanism of MIO-dependent enzymes from structure-guided directed evolution
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