2lm0
From Proteopedia
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| - | [[ | + | ==Solution structure of the AF4-AF9 complex== |
| + | <StructureSection load='2lm0' size='340' side='right' caption='[[2lm0]], [[NMR_Ensembles_of_Models | 10 NMR models]]' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[2lm0]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LM0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2LM0 FirstGlance]. <br> | ||
| + | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">AFF1, AF4, FEL, MLLT2, PBM1, MLLT3, AF9, YEATS3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2lm0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lm0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2lm0 RCSB], [http://www.ebi.ac.uk/pdbsum/2lm0 PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Mixed lineage leukemia (MLL) fusion proteins cause oncogenic transformation of hematopoietic cells by constitutive recruitment of elongation factors to HOX promoters, resulting in overexpression of target genes. The structural basis of transactivation by MLL fusion partners remains undetermined. We show that the ANC1 homology domain (AHD) of AF9, one of the most common MLL translocation partners, is intrinsically disordered and recruits multiple transcription factors through coupled folding and binding. We determined the structure of the AF9 AHD in complex with the elongation factor AF4 and show that aliphatic residues, which are conserved in each of the AF9 binding partners, form an integral part of the hydrophobic core of the complex. Nuclear magnetic resonance relaxation measurements show that AF9 retains significant dynamic behavior which may facilitate exchange between disordered partners. We propose that AF9 functions as a signaling hub that regulates transcription through dynamic recruitment of cofactors in normal hematopoiesis and in acute leukemia. | ||
| - | + | Leukemia Fusion Target AF9 Is an Intrinsically Disordered Transcriptional Regulator that Recruits Multiple Partners via Coupled Folding and Binding.,Leach BI, Kuntimaddi A, Schmidt CR, Cierpicki T, Johnson SA, Bushweller JH Structure. 2012 Dec 19. pii: S0969-2126(12)00426-1. doi:, 10.1016/j.str.2012.11.011. PMID:23260655<ref>PMID:23260655</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | == References == | |
| - | + | <references/> | |
| - | == | + | __TOC__ |
| - | + | </StructureSection> | |
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
| - | [[Category: Bushweller, J H | + | [[Category: Bushweller, J H]] |
| - | [[Category: Cierpicki, T | + | [[Category: Cierpicki, T]] |
| - | [[Category: Johnson, S A | + | [[Category: Johnson, S A]] |
| - | [[Category: Kuntimaddi, A | + | [[Category: Kuntimaddi, A]] |
| - | [[Category: Leach, B I | + | [[Category: Leach, B I]] |
| - | [[Category: Schmidt, C R | + | [[Category: Schmidt, C R]] |
[[Category: Intrinsically disordered]] | [[Category: Intrinsically disordered]] | ||
[[Category: Nuclear protein]] | [[Category: Nuclear protein]] | ||
Revision as of 08:15, 12 November 2014
Solution structure of the AF4-AF9 complex
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