1l2k

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[[Image:1l2k.gif|left|200px]]<br /><applet load="1l2k" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1l2k.gif|left|200px]]
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caption="1l2k, resolution 1.50&Aring;" />
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'''Neutron Structure Determination of Sperm Whale Met-Myoglobin at 1.5A Resolution.'''<br />
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{{Structure
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|PDB= 1l2k |SIZE=350|CAPTION= <scene name='initialview01'>1l2k</scene>, resolution 1.50&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ND4:AMMONIUM+CATION+WITH+D'>ND4</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene> and <scene name='pdbligand=DOD:DEUTERATED WATER'>DOD</scene>
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|ACTIVITY=
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|GENE=
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}}
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'''Neutron Structure Determination of Sperm Whale Met-Myoglobin at 1.5A Resolution.'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1L2K is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Physeter_catodon Physeter catodon] with <scene name='pdbligand=SO4:'>SO4</scene>, <scene name='pdbligand=ND4:'>ND4</scene>, <scene name='pdbligand=HEM:'>HEM</scene> and <scene name='pdbligand=DOD:'>DOD</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1L2K OCA].
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1L2K is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Physeter_catodon Physeter catodon]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1L2K OCA].
==Reference==
==Reference==
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Hydrogen and deuterium in myoglobin as seen by a neutron structure determination at 1.5 A resolution., Ostermann A, Tanaka I, Engler N, Niimura N, Parak FG, Biophys Chem. 2002 Mar 28;95(3):183-93. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12062378 12062378]
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Hydrogen and deuterium in myoglobin as seen by a neutron structure determination at 1.5 A resolution., Ostermann A, Tanaka I, Engler N, Niimura N, Parak FG, Biophys Chem. 2002 Mar 28;95(3):183-93. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12062378 12062378]
[[Category: Physeter catodon]]
[[Category: Physeter catodon]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: heme protein]]
[[Category: heme protein]]
[[Category: hydration structure]]
[[Category: hydration structure]]
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[[Category: hydrogen atoms]]
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[[Category: hydrogen atom]]
[[Category: neutron structure]]
[[Category: neutron structure]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:40:35 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:25:31 2008''

Revision as of 10:25, 20 March 2008

Image:1l2k.gif


PDB ID 1l2k

Drag the structure with the mouse to rotate
, resolution 1.50Å
Ligands: , , and
Coordinates: save as pdb, mmCIF, xml



Neutron Structure Determination of Sperm Whale Met-Myoglobin at 1.5A Resolution.


Overview

From the first days of protein neutron structure determination sperm whale myoglobin was an object under investigation [Nature 224 (1969) 143, J. Mol. Biol. 220 (1991) 381]. Nevertheless myoglobin is still of interest [Proc. Natl. Acad. Sci. USA 97 (2000) 3872]. The feasibility of the monochromatic neutron diffractometer BIX-3 at the JRR-3M reactor at the JAERI [J. Phys. Chem. Solids 60 (1999) 1623], to collect high-resolution diffraction data in a relatively short time stimulated us to repeat the structural determination of myoglobin. The structure of metmyoglobin has been determined up to a resolution of 1.5 A. The hydrogen atoms were replaced in part, by deuterium soaking the crystals for more than 10 years in D(2)O. A refinement of all atoms has been performed including the refinement of individual mean square displacements and occupancies of the exchangeable protons in backbone hydrogen bonds. A method is described to show clear negative scattering densities of the H atoms. Water molecules within the protein and on the molecule surface are shown. The exchangeability of H atoms is correlated with structural distribution and flexibility.

About this Structure

1L2K is a Single protein structure of sequence from Physeter catodon. Full crystallographic information is available from OCA.

Reference

Hydrogen and deuterium in myoglobin as seen by a neutron structure determination at 1.5 A resolution., Ostermann A, Tanaka I, Engler N, Niimura N, Parak FG, Biophys Chem. 2002 Mar 28;95(3):183-93. PMID:12062378

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