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3dp5

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[[Image:3dp5.png|left|200px]]
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==Crystal structure of Geobacter sulfurreducens OmcF with N-terminal Strep-tag II==
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<StructureSection load='3dp5' size='340' side='right' caption='[[3dp5]], [[Resolution|resolution]] 1.86&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3dp5]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Geobacter_sulfurreducens Geobacter sulfurreducens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3DP5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3DP5 FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">GSU2432 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=35554 Geobacter sulfurreducens])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3dp5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3dp5 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3dp5 RCSB], [http://www.ebi.ac.uk/pdbsum/3dp5 PDBsum]</span></td></tr>
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</table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/dp/3dp5_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The putative outer membrane c-type cytochrome OmcF from Geobacter sulfurreducens contains a single haem group and shows homology to soluble cytochromes c(6), a class of electron-transfer proteins that are typically found in cyanobacterial photosynthetic electron-transfer chains. OmcF was overexpressed heterologously in Escherichia coli as an N-terminal Strep-tag II fusion protein and isolated using streptactin-affinity chromatography followed by size-exclusion chromatography. The structure was solved by Fe SAD using data collected to a resolution of 1.86 A on a rotating copper-anode X-ray generator. In the crystal, packing interactions in one dimension were exclusively mediated through the Strep-tag II sequence. The tag and linker regions were in contact with three further monomers of OmcF, leading to a well defined electron-density map for this engineered and secondary-structure-free region of the molecule.
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{{STRUCTURE_3dp5| PDB=3dp5 | SCENE= }}
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Crystal packing of the c(6)-type cytochrome OmcF from Geobacter sulfurreducens is mediated by an N-terminal Strep-tag II.,Lukat P, Hoffmann M, Einsle O Acta Crystallogr D Biol Crystallogr. 2008 Sep;64(Pt 9):919-26. Epub 2008, Aug 13. PMID:18703839<ref>PMID:18703839</ref>
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===Crystal structure of Geobacter sulfurreducens OmcF with N-terminal Strep-tag II===
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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{{ABSTRACT_PUBMED_18703839}}
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== References ==
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<references/>
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==About this Structure==
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__TOC__
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[[3dp5]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Geobacter_sulfurreducens Geobacter sulfurreducens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3DP5 OCA].
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</StructureSection>
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==Reference==
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<ref group="xtra">PMID:018703839</ref><references group="xtra"/>
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[[Category: Geobacter sulfurreducens]]
[[Category: Geobacter sulfurreducens]]
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[[Category: Einsle, O.]]
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[[Category: Einsle, O]]
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[[Category: Hoffmann, M.]]
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[[Category: Hoffmann, M]]
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[[Category: Lukat, P.]]
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[[Category: Lukat, P]]
[[Category: C-type cytochrome]]
[[Category: C-type cytochrome]]
[[Category: Dissimilatory metal reduction]]
[[Category: Dissimilatory metal reduction]]

Revision as of 08:22, 12 November 2014

Crystal structure of Geobacter sulfurreducens OmcF with N-terminal Strep-tag II

3dp5, resolution 1.86Å

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