1l2x
From Proteopedia
| Line 1: | Line 1: | ||
| - | [[Image:1l2x.gif|left|200px]] | + | [[Image:1l2x.gif|left|200px]] |
| - | + | ||
| - | '''Atomic Resolution Crystal Structure of a Viral RNA Pseudoknot''' | + | {{Structure |
| + | |PDB= 1l2x |SIZE=350|CAPTION= <scene name='initialview01'>1l2x</scene>, resolution 1.25Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene> and <scene name='pdbligand=K:POTASSIUM ION'>K</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''Atomic Resolution Crystal Structure of a Viral RNA Pseudoknot''' | ||
| + | |||
==Overview== | ==Overview== | ||
| Line 7: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
| - | 1L2X is a [ | + | 1L2X is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1L2X OCA]. |
==Reference== | ==Reference== | ||
| - | Metal ions and flexibility in a viral RNA pseudoknot at atomic resolution., Egli M, Minasov G, Su L, Rich A, Proc Natl Acad Sci U S A. 2002 Apr 2;99(7):4302-7. Epub 2002 Mar 19. PMID:[http:// | + | Metal ions and flexibility in a viral RNA pseudoknot at atomic resolution., Egli M, Minasov G, Su L, Rich A, Proc Natl Acad Sci U S A. 2002 Apr 2;99(7):4302-7. Epub 2002 Mar 19. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11904368 11904368] |
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Egli, M.]] | [[Category: Egli, M.]] | ||
| Line 22: | Line 31: | ||
[[Category: flexibility]] | [[Category: flexibility]] | ||
[[Category: frameshifting]] | [[Category: frameshifting]] | ||
| - | [[Category: metal | + | [[Category: metal ion]] |
[[Category: pseudoknot]] | [[Category: pseudoknot]] | ||
[[Category: viral rna]] | [[Category: viral rna]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:25:34 2008'' |
Revision as of 10:25, 20 March 2008
| |||||||
| , resolution 1.25Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , and | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Atomic Resolution Crystal Structure of a Viral RNA Pseudoknot
Overview
Many pathogenic viruses use programmed -1 ribosomal frameshifting to regulate translation of their structural and enzymatic proteins from polycistronic mRNAs. Frameshifting is commonly stimulated by a pseudoknot located downstream from a slippery sequence, the latter positioned at the ribosomal A and P sites. We report here the structures of two crystal forms of the frameshifting RNA pseudoknot from beet western yellow virus at resolutions of 1.25 and 2.85 A. Because of the very high resolution of 1.25 A, ten mono- and divalent metal ions per asymmetric unit could be identified, giving insight into potential roles of metal ions in stabilizing the pseudoknot. A magnesium ion located at the junction of the two pseudoknot stems appears to play a crucial role in stabilizing the structure. Because the two crystal forms exhibit mostly unrelated packing interactions and local crystallographic disorder in the high-resolution form was resolvable, the two structures offer the most detailed view yet of the conformational preference and flexibility of an RNA pseudoknot.
About this Structure
1L2X is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
Metal ions and flexibility in a viral RNA pseudoknot at atomic resolution., Egli M, Minasov G, Su L, Rich A, Proc Natl Acad Sci U S A. 2002 Apr 2;99(7):4302-7. Epub 2002 Mar 19. PMID:11904368
Page seeded by OCA on Thu Mar 20 12:25:34 2008
Categories: Protein complex | Egli, M. | Minasov, G. | Rich, A. | Su, L. | K | MG | NA | Atomic resolution | Flexibility | Frameshifting | Metal ion | Pseudoknot | Viral rna
