1l3g
From Proteopedia
| Line 1: | Line 1: | ||
| - | [[Image:1l3g.jpg|left|200px]] | + | [[Image:1l3g.jpg|left|200px]] |
| - | + | ||
| - | '''NMR Structure of the DNA-binding Domain of Cell Cycle Protein, Mbp1(2-124) from Saccharomyces cerevisiae''' | + | {{Structure |
| + | |PDB= 1l3g |SIZE=350|CAPTION= <scene name='initialview01'>1l3g</scene> | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''NMR Structure of the DNA-binding Domain of Cell Cycle Protein, Mbp1(2-124) from Saccharomyces cerevisiae''' | ||
| + | |||
==Overview== | ==Overview== | ||
| Line 7: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
| - | 1L3G is a [ | + | 1L3G is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1L3G OCA]. |
==Reference== | ==Reference== | ||
| - | NMR structure of the DNA-binding domain of the cell cycle protein Mbp1 from Saccharomyces cerevisiae., Nair M, McIntosh PB, Frenkiel TA, Kelly G, Taylor IA, Smerdon SJ, Lane AN, Biochemistry. 2003 Feb 11;42(5):1266-73. PMID:[http:// | + | NMR structure of the DNA-binding domain of the cell cycle protein Mbp1 from Saccharomyces cerevisiae., Nair M, McIntosh PB, Frenkiel TA, Kelly G, Taylor IA, Smerdon SJ, Lane AN, Biochemistry. 2003 Feb 11;42(5):1266-73. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12564929 12564929] |
[[Category: Saccharomyces cerevisiae]] | [[Category: Saccharomyces cerevisiae]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| Line 22: | Line 31: | ||
[[Category: cell cycle]] | [[Category: cell cycle]] | ||
[[Category: mlu 1 cell cycle box binding protein]] | [[Category: mlu 1 cell cycle box binding protein]] | ||
| - | [[Category: winged helix-turn-helix | + | [[Category: winged helix-turn-helix protein]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:25:48 2008'' |
Revision as of 10:25, 20 March 2008
| |||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
NMR Structure of the DNA-binding Domain of Cell Cycle Protein, Mbp1(2-124) from Saccharomyces cerevisiae
Overview
The three-dimensional solution structure of the DNA-binding domain of Mlu-1 box binding protein (Mbp1) has been determined by multidimensional NMR spectroscopy. Mbp1 is a cell cycle transcription factor from Saccharomyces cerevisiae and consists of an N-terminal DNA-binding domain, a series of ankyrin repeats, and a heterodimerization domain at the C-terminus. A set of conformers comprising 19 refined structures was calculated via a molecular dynamics simulated annealing protocol using distance, dihedral angle, and residual dipolar coupling restraints derived from either double or triple resonance NMR experiments. The solution structure consists of a six-stranded beta-sheet segment folded against two pairs of alpha-helices in the topology of the winged helix-turn-helix family of proteins and is in agreement with the X-ray structures. In addition, the solution structure shows that the C-terminal tail region of this domain folds back and makes specific interactions with the N-terminal beta-strand of the protein. This C-terminal region is essential for full DNA-binding activity but appears in the X-ray structure to be disordered. The fold-back structure extends the region of positive electrostatic potential, and this may enhance the nonspecific contribution to binding by favorable electrostatic interactions with the DNA backbone.
About this Structure
1L3G is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
Reference
NMR structure of the DNA-binding domain of the cell cycle protein Mbp1 from Saccharomyces cerevisiae., Nair M, McIntosh PB, Frenkiel TA, Kelly G, Taylor IA, Smerdon SJ, Lane AN, Biochemistry. 2003 Feb 11;42(5):1266-73. PMID:12564929
Page seeded by OCA on Thu Mar 20 12:25:48 2008
