1l9u
From Proteopedia
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| - | [[Image:1l9u.gif|left|200px]] | + | [[Image:1l9u.gif|left|200px]] |
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| - | '''THERMUS AQUATICUS RNA POLYMERASE HOLOENZYME AT 4 A RESOLUTION''' | + | {{Structure |
| + | |PDB= 1l9u |SIZE=350|CAPTION= <scene name='initialview01'>1l9u</scene>, resolution 4.0Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene> and <scene name='pdbligand=ZN:ZINC ION'>ZN</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/DNA-directed_RNA_polymerase DNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.6 2.7.7.6] | ||
| + | |GENE= RPOD ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=271 Thermus aquaticus]) | ||
| + | }} | ||
| + | |||
| + | '''THERMUS AQUATICUS RNA POLYMERASE HOLOENZYME AT 4 A RESOLUTION''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1L9U is a [ | + | 1L9U is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Thermus_aquaticus Thermus aquaticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1L9U OCA]. |
==Reference== | ==Reference== | ||
| - | Structural basis of transcription initiation: RNA polymerase holoenzyme at 4 A resolution., Murakami KS, Masuda S, Darst SA, Science. 2002 May 17;296(5571):1280-4. PMID:[http:// | + | Structural basis of transcription initiation: RNA polymerase holoenzyme at 4 A resolution., Murakami KS, Masuda S, Darst SA, Science. 2002 May 17;296(5571):1280-4. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12016306 12016306] |
[[Category: DNA-directed RNA polymerase]] | [[Category: DNA-directed RNA polymerase]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
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[[Category: helix-turn-helix]] | [[Category: helix-turn-helix]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:28:19 2008'' |
Revision as of 10:28, 20 March 2008
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| , resolution 4.0Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | and | ||||||
| Gene: | RPOD (Thermus aquaticus) | ||||||
| Activity: | DNA-directed RNA polymerase, with EC number 2.7.7.6 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
THERMUS AQUATICUS RNA POLYMERASE HOLOENZYME AT 4 A RESOLUTION
Overview
The crystal structure of the initiating form of Thermus aquaticus RNA polymerase, containing core RNA polymerase (alpha2betabeta'omega) and the promoter specificity sigma subunit, has been determined at 4 angstrom resolution. Important structural features of the RNA polymerase and their roles in positioning sigma within the initiation complex are delineated, as well as the role played by sigma in modulating the opening of the RNA polymerase active-site channel. The two carboxyl-terminal domains of sigma are separated by 45 angstroms on the surface of the RNA polymerase, but are linked by an extended loop. The loop winds near the RNA polymerase active site, where it may play a role in initiating nucleotide substrate binding, and out through the RNA exit channel. The advancing RNA transcript must displace the loop, leading to abortive initiation and ultimately to sigma release.
About this Structure
1L9U is a Protein complex structure of sequences from Thermus aquaticus. Full crystallographic information is available from OCA.
Reference
Structural basis of transcription initiation: RNA polymerase holoenzyme at 4 A resolution., Murakami KS, Masuda S, Darst SA, Science. 2002 May 17;296(5571):1280-4. PMID:12016306
Page seeded by OCA on Thu Mar 20 12:28:19 2008
