1laj
From Proteopedia
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| - | [[Image:1laj.gif|left|200px]] | + | [[Image:1laj.gif|left|200px]] |
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| - | '''The Structure of Tomato Aspermy Virus by X-Ray Crystallography''' | + | {{Structure |
| + | |PDB= 1laj |SIZE=350|CAPTION= <scene name='initialview01'>1laj</scene>, resolution 3.4Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene> and <scene name='pdbligand=MG:MAGNESIUM ION'>MG</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''The Structure of Tomato Aspermy Virus by X-Ray Crystallography''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1LAJ is a [ | + | 1LAJ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Tomato_aspermy_virus Tomato aspermy virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LAJ OCA]. |
==Reference== | ==Reference== | ||
| - | The structure of tomato aspermy virus by X-ray crystallography., Lucas RW, Larson SB, Canady MA, McPherson A, J Struct Biol. 2002 Aug;139(2):90-102. PMID:[http:// | + | The structure of tomato aspermy virus by X-ray crystallography., Lucas RW, Larson SB, Canady MA, McPherson A, J Struct Biol. 2002 Aug;139(2):90-102. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12406691 12406691] |
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Tomato aspermy virus]] | [[Category: Tomato aspermy virus]] | ||
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[[Category: MG]] | [[Category: MG]] | ||
[[Category: PO4]] | [[Category: PO4]] | ||
| - | [[Category: anti-parallel beta | + | [[Category: anti-parallel beta sheet]] |
[[Category: disulphide bridge]] | [[Category: disulphide bridge]] | ||
[[Category: icosahedral virus]] | [[Category: icosahedral virus]] | ||
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[[Category: t=3 icosahedral virus]] | [[Category: t=3 icosahedral virus]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:28:35 2008'' |
Revision as of 10:28, 20 March 2008
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| , resolution 3.4Å | |||||||
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| Ligands: | and | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
The Structure of Tomato Aspermy Virus by X-Ray Crystallography
Overview
The three-dimensional structure of tomato aspermy virus (TAV) has been solved by X-ray crystallography and refined to an R factor of 0.218 for 3.4-40 A data (effective resolution of 4A). Molecular replacement, using cucumber mosaic virus (Smith et al., 2000), provided phases for the initial maps used for model building. The coat protein of the 280 A diameter virion has the canonical "Swiss roll" beta-barrel topology with a distinctive amino-terminal alpha-helix directed into the interior of the virus where it interacts with encapsidated RNA. The N-terminal helices are joined to the beta-barrels of protein subunits by extended polypeptides of six amino acids, which serve as flexible hinges allowing movement of the helices in response to local RNA distribution. Segments of three nucleotides of partially disordered RNA interact with the capsid, primarily through arginine residues, at interfaces between A and B subunits. Side chains of cys64 and cys106 form the first disulfide observed in a cucumovirus, including a unique cysteine, 106, in a region otherwise conserved. A positive ion, putatively modeled as a Mg(+)ion, lies on the quasi-threefold axis surrounded by three quasi-symmetric glutamate 175 side chains.
About this Structure
1LAJ is a Single protein structure of sequence from Tomato aspermy virus. Full crystallographic information is available from OCA.
Reference
The structure of tomato aspermy virus by X-ray crystallography., Lucas RW, Larson SB, Canady MA, McPherson A, J Struct Biol. 2002 Aug;139(2):90-102. PMID:12406691
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