1lag
From Proteopedia
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| - | [[Image:1lag.gif|left|200px]] | + | [[Image:1lag.gif|left|200px]] |
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| - | '''STRUCTURAL BASES FOR MULTIPLE LIGAND SPECIFICITY OF THE PERIPLASMIC LYSINE-, ARGININE-, ORNITHINE-BINDING PROTEIN''' | + | {{Structure |
| + | |PDB= 1lag |SIZE=350|CAPTION= <scene name='initialview01'>1lag</scene>, resolution 2.06Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=HIS:HISTIDINE'>HIS</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''STRUCTURAL BASES FOR MULTIPLE LIGAND SPECIFICITY OF THE PERIPLASMIC LYSINE-, ARGININE-, ORNITHINE-BINDING PROTEIN''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1LAG is a [ | + | 1LAG is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Salmonella_typhimurium Salmonella typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LAG OCA]. |
==Reference== | ==Reference== | ||
| - | Structural basis for multiple ligand specificity of the periplasmic lysine-, arginine-, ornithine-binding protein., Oh BH, Ames GF, Kim SH, J Biol Chem. 1994 Oct 21;269(42):26323-30. PMID:[http:// | + | Structural basis for multiple ligand specificity of the periplasmic lysine-, arginine-, ornithine-binding protein., Oh BH, Ames GF, Kim SH, J Biol Chem. 1994 Oct 21;269(42):26323-30. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/7929349 7929349] |
[[Category: Salmonella typhimurium]] | [[Category: Salmonella typhimurium]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: amino acid transport]] | [[Category: amino acid transport]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:28:32 2008'' |
Revision as of 10:28, 20 March 2008
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| , resolution 2.06Å | |||||||
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| Coordinates: | save as pdb, mmCIF, xml | ||||||
STRUCTURAL BASES FOR MULTIPLE LIGAND SPECIFICITY OF THE PERIPLASMIC LYSINE-, ARGININE-, ORNITHINE-BINDING PROTEIN
Overview
The substrate-binding site of a protein with multiple specificity should satisfy geometric and energetic complementarity for several different substrates. The structural basis of the multiple ligand specificity of the periplasmic lysine-, arginine-, ornithine-binding protein (LAO) was investigated by determining and analyzing the structures of the protein unliganded and liganded with each of the three high-affinity ligands (L-lysine, L-arginine, and L-ornithine) and with one low-affinity ligand (L-histidine). The geometric complementarity is achieved primarily by virtue of the large size of the ligand-binding site which can accommodate the maximum common volume of the four ligands plus three water molecules. The optimization of energetic complementarity is achieved by the relocation of protein-bound water molecules and by the movement of the Asp-11 side chain. The structure of the LAO-histidine complex indicates that the 30-fold reduced affinity of the protein for histidine is primarily due to unavailability of one ionic interaction of the histidine side chain with the protein which is present in the other three complexes.
About this Structure
1LAG is a Single protein structure of sequence from Salmonella typhimurium. Full crystallographic information is available from OCA.
Reference
Structural basis for multiple ligand specificity of the periplasmic lysine-, arginine-, ornithine-binding protein., Oh BH, Ames GF, Kim SH, J Biol Chem. 1994 Oct 21;269(42):26323-30. PMID:7929349
Page seeded by OCA on Thu Mar 20 12:28:32 2008
