1lci
From Proteopedia
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| - | [[Image:1lci.jpg|left|200px]] | + | [[Image:1lci.jpg|left|200px]] |
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| - | '''FIREFLY LUCIFERASE''' | + | {{Structure |
| + | |PDB= 1lci |SIZE=350|CAPTION= <scene name='initialview01'>1lci</scene>, resolution 2.0Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Photinus-luciferin_4-monooxygenase_(ATP-hydrolyzing) Photinus-luciferin 4-monooxygenase (ATP-hydrolyzing)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.13.12.7 1.13.12.7] | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''FIREFLY LUCIFERASE''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1LCI is a [ | + | 1LCI is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Photinus_pyralis Photinus pyralis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LCI OCA]. |
==Reference== | ==Reference== | ||
| - | Crystal structure of firefly luciferase throws light on a superfamily of adenylate-forming enzymes., Conti E, Franks NP, Brick P, Structure. 1996 Mar 15;4(3):287-98. PMID:[http:// | + | Crystal structure of firefly luciferase throws light on a superfamily of adenylate-forming enzymes., Conti E, Franks NP, Brick P, Structure. 1996 Mar 15;4(3):287-98. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8805533 8805533] |
[[Category: Photinus pyralis]] | [[Category: Photinus pyralis]] | ||
[[Category: Photinus-luciferin 4-monooxygenase (ATP-hydrolyzing)]] | [[Category: Photinus-luciferin 4-monooxygenase (ATP-hydrolyzing)]] | ||
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[[Category: photoprotein]] | [[Category: photoprotein]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:29:17 2008'' |
Revision as of 10:29, 20 March 2008
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| , resolution 2.0Å | |||||||
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| Activity: | Photinus-luciferin 4-monooxygenase (ATP-hydrolyzing), with EC number 1.13.12.7 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
FIREFLY LUCIFERASE
Overview
BACKGROUND. Firefly luciferase is a 62 kDa protein that catalyzes the production of light. In the presence of MgATP and molecular oxygen, the enzyme oxidizes its substrate, firefly luciferin, emitting yellow-green light. The reaction proceeds through activation of the substrate to form an adenylate intermediate. Firefly luciferase shows extensive sequence homology with a number of enzymes that utilize ATP in adenylation reactions. RESULTS. We have determined the crystal structure of firefly luciferase at 2.0 A resolution. The protein is folded into two compact domains. The large N-terminal domain consists of a beta-barrel and two beta-sheets. The sheets are flanked by alpha-helices to form an alphabetaalphabetaalpha five-layered structure. The C-terminal portion of the molecule forms a distinct domain, which is separated from the N-terminal domain by a wide cleft. CONCLUSIONS. Firefly luciferase is the first member of a superfamily of homologous enzymes, which includes acyl-coenzyme A ligases and peptide synthetases, to have its structure characterized. The residues conserved within the superfamily are located on the surfaces of the two domains on either side of the cleft, but are too far apart to interact simultaneously with the substrates. This suggests that the two domains will close in the course of the reaction. Firefly luciferase has a novel structural framework for catalyzing adenylate-forming reactions.
About this Structure
1LCI is a Single protein structure of sequence from Photinus pyralis. Full crystallographic information is available from OCA.
Reference
Crystal structure of firefly luciferase throws light on a superfamily of adenylate-forming enzymes., Conti E, Franks NP, Brick P, Structure. 1996 Mar 15;4(3):287-98. PMID:8805533
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