1lcf

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[[Image:1lcf.gif|left|200px]]<br /><applet load="1lcf" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1lcf.gif|left|200px]]
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caption="1lcf, resolution 2.0&Aring;" />
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'''CRYSTAL STRUCTURE OF COPPER-AND OXALATE-SUBSTITUTED HUMAN LACTOFERRIN AT 2.0 ANGSTROMS RESOLUTION'''<br />
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{{Structure
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|PDB= 1lcf |SIZE=350|CAPTION= <scene name='initialview01'>1lcf</scene>, resolution 2.0&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=CO3:CARBONATE+ION'>CO3</scene> and <scene name='pdbligand=OXL:OXALATE ION'>OXL</scene>
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|ACTIVITY=
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|GENE=
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}}
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'''CRYSTAL STRUCTURE OF COPPER-AND OXALATE-SUBSTITUTED HUMAN LACTOFERRIN AT 2.0 ANGSTROMS RESOLUTION'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1LCF is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=NAG:'>NAG</scene>, <scene name='pdbligand=CU:'>CU</scene>, <scene name='pdbligand=CO3:'>CO3</scene> and <scene name='pdbligand=OXL:'>OXL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LCF OCA].
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1LCF is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LCF OCA].
==Reference==
==Reference==
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Structure of copper- and oxalate-substituted human lactoferrin at 2.0 A resolution., Smith CA, Anderson BF, Baker HM, Baker EN, Acta Crystallogr D Biol Crystallogr. 1994 May 1;50(Pt 3):302-16. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15299444 15299444]
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Structure of copper- and oxalate-substituted human lactoferrin at 2.0 A resolution., Smith CA, Anderson BF, Baker HM, Baker EN, Acta Crystallogr D Biol Crystallogr. 1994 May 1;50(Pt 3):302-16. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15299444 15299444]
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: iron transport]]
[[Category: iron transport]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:43:40 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:29:14 2008''

Revision as of 10:29, 20 March 2008

Image:1lcf.gif


PDB ID 1lcf

Drag the structure with the mouse to rotate
, resolution 2.0Å
Ligands: , , and
Coordinates: save as pdb, mmCIF, xml



CRYSTAL STRUCTURE OF COPPER-AND OXALATE-SUBSTITUTED HUMAN LACTOFERRIN AT 2.0 ANGSTROMS RESOLUTION


Contents

Overview

The three-dimensional structure of human dicupric monooxalate lactoferrin, Cu(2)oxLf, has been determined to 2.0 A resolution, using X-ray diffraction data collected by diffractometry to 2.5 A resolution, and oscillation photography on a synchrotron source to 2.0 A resolution. Difference electron-density maps calculated between Cu(2)oxLf and both dicupric lactoferrin, Cu(2)Lf, and diferric lactoferrin, Fe(2)Lf, showed that the oxalate had replaced a carbonate in the C-terminal binding site, and that, relative to Cu(2)Lf, there were no significant differences in the N-terminal site. The structure was then refined crystallographically by restrained least-squares methods. The final model, in which the r.m.s. deviation in bond distances is 0.017 A, contains 5314 protein atoms (691 residues), two Cu(2+) ions, one bicarbonate ion, one oxalate ion, 325 solvent molecules and one sugar residue. The crystallographic R factor of 0.193 is for 46 134 reflections in the range 8.0 to 2.0 A resolution. The oxalate ion is coordinated to copper in a 1,2-bidentate fashion, and the added bulk of the anion results in the rearrangement of the side chains of nearby arginine and tyrosine residues. No other major alterations in the molecule can be observed, the overall protein structure being the same as that for Cu(2)Lf and Fe(2)Lf.

Disease

Known disease associated with this structure: Deafness, autosomal dominant 1 OMIM:[602121]

About this Structure

1LCF is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Structure of copper- and oxalate-substituted human lactoferrin at 2.0 A resolution., Smith CA, Anderson BF, Baker HM, Baker EN, Acta Crystallogr D Biol Crystallogr. 1994 May 1;50(Pt 3):302-16. PMID:15299444

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